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Literature summary extracted from

  • Hong, S.H.; Ngo, H.P.; Kang, L.W.; Oh, D.K.
    Characterization of alcohol dehydrogenase from Kangiella koreensis and its application to production of all-trans-retinol (2015), Biotechnol. Lett., 37, 849-856.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.1 synthesis under optimized conditions, the enzyme produces 600 mg all-trans-retinol per l after 3 h, with a conversion yield of 27.3% (w/w) and a productivity of 200 mg per l and h Kangiella koreensis
1.1.1.105 synthesis under optimized conditions, the enzyme produces 600 mg all-trans-retinol per l after 3 h, with a conversion yield of 27.3% (w/w) and a productivity of 200 mg per l and h Kangiella koreensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expression in Escherichia coli Kangiella koreensis
1.1.1.105 expressed in Escherichia coli ER2566 cells Kangiella koreensis
1.1.1.105 expression in Escherichia coli Kangiella koreensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.105 additional information the enzyme is not influenced by EDTA Kangiella koreensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.105 0.0018
-
 all-trans-retinal at pH 6.5 and 60°C Kangiella koreensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.105 additional information the enzyme activity is not affected by monovalent or divalent cations Kangiella koreensis
1.1.1.105 Zn2+ contains Zn2+ Kangiella koreensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 39900
-
-
 Kangiella koreensis
1.1.1.1 40000
-
-
 Kangiella koreensis
1.1.1.105 39900
-
-
 Kangiella koreensis
1.1.1.105 40000
-
-
 Kangiella koreensis
1.1.1.105 40000
-
 2 * 40000, SDS-PAGE Kangiella koreensis
1.1.1.105 80000
-
 gel filtration Kangiella koreensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.105 all-trans-retinal + NADH + H+ Kangiella koreensis
-
 all-trans-retinol + NAD+
-
 r
1.1.1.105 all-trans-retinal + NADH + H+ Kangiella koreensis SW-125
-
 all-trans-retinol + NAD+
-
 r
1.1.1.105 all-trans-retinol + NAD+ Kangiella koreensis
-
 all-trans-retinal + NADH + H+
-
 r
1.1.1.105 all-trans-retinol + NAD+ Kangiella koreensis SW-125
-
 all-trans-retinal + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Kangiella koreensis C7R702
-
-
1.1.1.105 Kangiella koreensis
-
-
-
1.1.1.105 Kangiella koreensis C7R702
-
-
1.1.1.105 Kangiella koreensis SW-125
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.105 immobilized metal ion affinity chromatography Kangiella koreensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 79.6
-
 substrate butan-1-ol, pH 6.5, 60°C Kangiella koreensis
1.1.1.105 0.0213
-
 pH 6.5, 60°C Kangiella koreensis
1.1.1.105 0.0213
-
 with all-trans-retinal as substrate, at pH 6.5 and 60°C Kangiella koreensis
1.1.1.105 0.024
-
 with all-trans-retinol as substrate, at pH 6.5 and 60°C Kangiella koreensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 acetaldehyde + NADH + H+ 83.2% of the activity with butan-1-ol Kangiella koreensis ethanol + NAD+
-
 ?
1.1.1.1 all-trans retinal + NADH + H+ 26.8% of the activity with butan-1-ol Kangiella koreensis all-trans retinol + NAD+
-
 ?
1.1.1.1 butan-1-ol + NAD+
-
 Kangiella koreensis butanal + NADH + H+
-
 ?
1.1.1.1 hexan-1-ol + NAD+ 77.1% of the activity with butan-1-ol Kangiella koreensis hexanal + NADH + H+
-
 ?
1.1.1.1 additional information enzyme is an alcohol dehydrogenase with additional activity for all-trans-retinol, reaction of EC 1.1.1.184 Kangiella koreensis ?
-
 ?
1.1.1.1 propan-1-ol + NAD+ 64.3% of the activity with butan-1-ol Kangiella koreensis propanal + NADH + H+
-
 ?
1.1.1.105 all-trans retinal + NADH + H+ 26.8% of the activity with butan-1-ol Kangiella koreensis all-trans retinol + NAD+
-
 ?
1.1.1.105 all-trans-retinal + NADH + H+
-
 Kangiella koreensis all-trans-retinol + NAD+
-
 r
1.1.1.105 all-trans-retinal + NADH + H+ 26% activity compared to butyl alcohol Kangiella koreensis all-trans-retinol + NAD+
-
 r
1.1.1.105 all-trans-retinal + NADH + H+
-
 Kangiella koreensis SW-125 all-trans-retinol + NAD+
-
 r
1.1.1.105 all-trans-retinal + NADH + H+ 26% activity compared to butyl alcohol Kangiella koreensis SW-125 all-trans-retinol + NAD+
-
 r
1.1.1.105 all-trans-retinol + NAD+
-
 Kangiella koreensis all-trans-retinal + NADH + H+
-
 r
1.1.1.105 all-trans-retinol + NAD+ 30.2% activity compared to butyl alcohol Kangiella koreensis all-trans-retinal + NADH + H+
-
 ?
1.1.1.105 all-trans-retinol + NAD+
-
 Kangiella koreensis SW-125 all-trans-retinal + NADH + H+
-
 r
1.1.1.105 all-trans-retinol + NAD+ 30.2% activity compared to butyl alcohol Kangiella koreensis SW-125 all-trans-retinal + NADH + H+
-
 ?
1.1.1.105 butyl alcohol + NAD+ 100% activity Kangiella koreensis butanal + NADH + H+
-
 ?
1.1.1.105 butyl alcohol + NAD+ 100% activity Kangiella koreensis SW-125 butanal + NADH + H+
-
 ?
1.1.1.105 additional information enzyme is an alcohol dehydrogenase, EC 1.1.1.1, with activity for all-trans-retinol using NAD+ as a cofactor Kangiella koreensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 ? x * 40000, SDS-PAGE, x * 39900, calculated Kangiella koreensis
1.1.1.105 ? x * 40000, SDS-PAGE, x * 39900, calculated Kangiella koreensis
1.1.1.105 homodimer 2 * 40000, SDS-PAGE Kangiella koreensis
1.1.1.105 homodimer 2 * 39900, cslculated from amino acid sequence Kangiella koreensis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.105 ADH
-
 Kangiella koreensis
1.1.1.105 alcohol dehydrogenase
-
 Kangiella koreensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.105 60
-
-
 Kangiella koreensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.105 0.028
-
 all-trans-retinal at pH 6.5 and 60°C Kangiella koreensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.105 6.5
-
-
 Kangiella koreensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Kangiella koreensis
1.1.1.1 NADH
-
 Kangiella koreensis
1.1.1.105 NAD+
-
 Kangiella koreensis
1.1.1.105 NADH
-
 Kangiella koreensis
1.1.1.105 NADP+
-
 Kangiella koreensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.105 15.7
-
 all-trans-retinal at pH 6.5 and 60°C Kangiella koreensis