EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.15 | expression in Escherichia coli | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.15 | E202L | mutation in NXXE motif, increase in the Km for MgATP of 52fold, and a decrease in the kcat value of around 200fold | Homo sapiens |
2.7.1.15 | N199L | mutation in NXXE motif, increase in the Km for MgATP- by about 68fold, a decrease in the kcat between 573- and 1036fold and almost no change in the Km for D-ribose. No substrate inhibition by D-ribose | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.15 | ATP | - |
Homo sapiens | |
2.7.1.15 | D-ribose | substrate inhibition | Homo sapiens | |
2.7.1.15 | EDTA | complete inhibition | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.15 | 0.029 | - |
ATP | wild-type, presence of Mn2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 0.08 | - |
D-ribose | mutant E202L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 0.12 | - |
D-ribose | mutant N199L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 0.12 | - |
D-ribose | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 0.16 | - |
ATP | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 5.73 | - |
ATP | mutant E202L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 7.48 | - |
ATP | mutant N199L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.15 | Cd2+ | may partly support activity | Homo sapiens | |
2.7.1.15 | Co2+ | about 60% of the activity with Mn2+ | Homo sapiens | |
2.7.1.15 | Mg2+ | about 70% of the activity with Mn2+ | Homo sapiens | |
2.7.1.15 | Mn2+ | best activator, optimum concentration 0.1 mM | Homo sapiens | |
2.7.1.15 | Ni2+ | may partly support activity | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.15 | Homo sapiens | Q9H477 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.15 | ATP + D-ribose | - |
Homo sapiens | ADP + D-ribose 5-phosphate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.15 | 0.01 | - |
ATP | mutant N199L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 0.01 | - |
D-ribose | mutant N199L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 0.04 | - |
ATP | mutant E202L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 0.04 | - |
D-ribose | mutant E202L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 7.3 | - |
ATP | wild-type, presence of Mn2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 9.8 | - |
ATP | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 10.4 | - |
D-ribose | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.15 | 10.3 | - |
ATP | wild-type, presence of Mn2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 16.9 | - |
ATP | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.15 | 0.0013 | - |
ATP | mutant N199L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 0.007 | - |
ATP | mutant E202L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 0.08 | - |
D-ribose | mutant N199L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 0.5 | - |
D-ribose | mutant E202L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 60 | - |
ATP | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 90 | - |
D-ribose | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
2.7.1.15 | 250 | - |
ATP | wild-type, presence of Mn2+, pH 7.2, 37°C | Homo sapiens |