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Literature summary extracted from

  • Zhang, X.; Eser, B.E.; Chanani, P.K.; Begley, T.P.; Ealick, S.E.
    Structural Basis for iron-mediated sulfur transfer in archael and yeast thiazole synthases (2016), Biochemistry, 55, 1826-1838.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.10 expressed in Escherichia coli BL21(DE3) cells Methanocaldococcus jannaschii
2.8.1.10 expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae
2.8.1.10 expressed in Escherichia coli BL21(DE3) cells Methanotorris igneus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.1.10 C205S variant with a bound glycine imine intermediate, hanging drop vapor diffusion method, using 25% (w/v) PEG1500, 0.0125 M succinic acid, 0.044 M sodium dihydrogen phosphate, and 0.044 M glycine, pH 8.5 Saccharomyces cerevisiae
2.8.1.10 wild type enzyme complexed with ADP-ribulose, hanging drop vapor diffusion method, using 1.05 M potassium/sodium tartrate, 0.1 M CHES/sodium hydroxide, pH 9.5 and 0.2 M lithium sulfate Methanocaldococcus jannaschii
2.8.1.10 wild type enzyme with bound glycine imine intermediate and iron, hanging drop vapor diffusion method, using 1.0 M sodium citrate, 0.1 M CHES/sodium hydroxide, pH 9.5 Methanotorris igneus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.10 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] Methanocaldococcus jannaschii
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
?
2.8.1.10 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] Saccharomyces cerevisiae
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
?
2.8.1.10 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] Methanotorris igneus
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.10 Methanocaldococcus jannaschii
-
-
-
2.8.1.10 Methanotorris igneus
-
-
-
2.8.1.10 Saccharomyces cerevisiae P32318
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.10 Ni-NTA column chromatography and Superdex 200 gel filtration Methanocaldococcus jannaschii
2.8.1.10 Ni-NTA column chromatography and Superdex 200 gel filtration Saccharomyces cerevisiae
2.8.1.10 Ni-NTA column chromatography and Superdex 200 gel filtration Methanotorris igneus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.10 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
Methanocaldococcus jannaschii 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
?
2.8.1.10 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
Saccharomyces cerevisiae 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
?
2.8.1.10 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
Methanotorris igneus 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
2.8.1.10 Thi4
-
Methanocaldococcus jannaschii
2.8.1.10 Thi4
-
Saccharomyces cerevisiae
2.8.1.10 Thi4
-
Methanotorris igneus