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Literature summary extracted from

  • McGresham, M.S.; Lovingshimer, M.; Reinhart, G.D.
    Allosteric regulation in phosphofructokinase from the extreme thermophile Thermus thermophilus (2014), Biochemistry, 53, 270-278.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.11
-
 Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.11 L313W variant behaves similarly to wild type protein Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 fructose 6-phosphate
-
 Thermus thermophilus
2.7.1.11 MgADP- addition of MgADP has little effect the specific activity of the enzyme, but diminishes the homotropic cooperativity of fructose 6-phosphate binding, reducing the Hill numbers from 1.6 to 1 Thermus thermophilus
2.7.1.11 phosphoenolpyruvate addition leads to a decease in fructose 6-phosphate binding affinity and in heterotropically induced homotropic cooperativity in fructose 6-phosphate binding, the Hill numbers increase from 1.6 to above 2.5 without decrease in specific activity Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 additional information
-
 additional information allosteric regulation. At saturating fructose 6-phoshate (5 mM), the Hill number for MgATP binding is 0.8 Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Thermus thermophilus P21777
-
-
2.7.1.11 Thermus thermophilus DSM 579 P21777
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 41
-
 pH 8.0, 25°C Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Thermus thermophilus ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Thermus thermophilus DSM 579 ADP + D-fructose 1,6-bisphosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.11 0.0004
-
 MgADP- pH 8.0, 25°C Thermus thermophilus
2.7.1.11 0.0016
-
 phosphoenolpyruvate pH 8.0, 25°C Thermus thermophilus
2.7.1.11 0.027
-
 fructose 6-phosphate pH 8.0, 25°C Thermus thermophilus