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Literature summary extracted from

  • Ishii, Y.; Narimatsu, Y.; Iwasaki, Y.; Arai, N.; Kino, K.; Kirimura, K.
    Reversible and nonoxidative gamma-resorcylic acid decarboxylase: Characterization and gene cloning of a novel enzyme catalyzing carboxylation of resorcinol, 1,3-dihydroxybenzene, from Rhizobium radiobacter (2004), Biochem. Biophys. Res. Commun., 324, 611-620.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.103 Ag+ 1 mM, 0% of initial activity for decarboxylation reaction, 12% of initial activity for carboxylation reaction Agrobacterium tumefaciens
4.1.1.103 diethyl dicarbonate 1 mM, 66% of initial activity for decarboxylation reaction, 62% of initial activity for carboxylation reaction Agrobacterium tumefaciens
4.1.1.103 Hg2+ 1 mM, 21% of initial activity for decarboxylation reaction, 94% of initial activity for carboxylation reaction Agrobacterium tumefaciens
4.1.1.103 additional information not inhibitory: presence of oxygen Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.103 7.1
-
 2,6-dihydroxybenzoate pH 7.0, 40°C Agrobacterium tumefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.103 Mg2+ 1 mM, 132% of initial activity for decarboxylation reaction, 106% of initial activity for carboxylation reaction Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.103 38000
-
 4 * 38000, SDS-PAGE Agrobacterium tumefaciens
4.1.1.103 130000
-
 gel filtration Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.103 1,3-dihydroxybenzene + CO2 Agrobacterium tumefaciens
-
 2,6-dihydroxybenzoate
-
 r
4.1.1.103 1,3-dihydroxybenzene + CO2 Agrobacterium tumefaciens WU-0108
-
 2,6-dihydroxybenzoate
-
 r
4.1.1.103 2,3-Dihydroxybenzoate Agrobacterium tumefaciens
-
 Catechol + CO2
-
 r
4.1.1.103 2,3-Dihydroxybenzoate Agrobacterium tumefaciens WU-0108
-
 Catechol + CO2
-
 r
4.1.1.103 2,6-dihydroxybenzoate Agrobacterium tumefaciens
-
 1,3-dihydroxybenzene + CO2
-
 r
4.1.1.103 2,6-dihydroxybenzoate Agrobacterium tumefaciens WU-0108
-
 1,3-dihydroxybenzene + CO2
-
 r
4.1.1.103 catechol + CO2 Agrobacterium tumefaciens
-
 2,3-dihydroxybenzoate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.103 Agrobacterium tumefaciens Q60FX6
-
-
4.1.1.103 Agrobacterium tumefaciens WU-0108 Q60FX6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.103 1.57
-
 carboxylation reaction, pH 7.0, 40°C Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.103 1,3-dihydroxybenzene + CO2
-
 Agrobacterium tumefaciens 2,6-dihydroxybenzoate
-
 ?
4.1.1.103 1,3-dihydroxybenzene + CO2
-
 Agrobacterium tumefaciens 2,6-dihydroxybenzoate
-
 r
4.1.1.103 1,3-dihydroxybenzene + CO2
-
 Agrobacterium tumefaciens WU-0108 2,6-dihydroxybenzoate
-
 ?
4.1.1.103 1,3-dihydroxybenzene + CO2
-
 Agrobacterium tumefaciens WU-0108 2,6-dihydroxybenzoate
-
 r
4.1.1.103 2,3-Dihydroxybenzoate
-
 Agrobacterium tumefaciens Catechol + CO2
-
 r
4.1.1.103 2,3-Dihydroxybenzoate
-
 Agrobacterium tumefaciens WU-0108 Catechol + CO2
-
 r
4.1.1.103 2,6-dihydroxybenzoate
-
 Agrobacterium tumefaciens 1,3-dihydroxybenzene + CO2
-
 ?
4.1.1.103 2,6-dihydroxybenzoate
-
 Agrobacterium tumefaciens 1,3-dihydroxybenzene + CO2
-
 r
4.1.1.103 2,6-dihydroxybenzoate
-
 Agrobacterium tumefaciens WU-0108 1,3-dihydroxybenzene + CO2
-
 ?
4.1.1.103 2,6-dihydroxybenzoate
-
 Agrobacterium tumefaciens WU-0108 1,3-dihydroxybenzene + CO2
-
 r
4.1.1.103 catechol + CO2
-
 Agrobacterium tumefaciens 2,3-dihydroxybenzoate
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.1.103 tetramer 4 * 38000, SDS-PAGE Agrobacterium tumefaciens

Synonyms

EC Number Synonyms Comment Organism
4.1.1.103 Rdc
-
 Agrobacterium tumefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.103 60
-
-
 Agrobacterium tumefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.103 40
-
 stable up to Agrobacterium tumefaciens
4.1.1.103 50
-
 30 min, 60% residual activity Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.103 20
-
 2,6-dihydroxybenzoate pH 7.0, 40°C Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.103 7
-
-
 Agrobacterium tumefaciens