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Literature summary extracted from
- Phycobilin biosynthesis: Reductant requirements and product identification for heme oxygenase from Cyanidium caldarium (1995), Arch. Biochem. Biophys., 320, 182-194.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.20 | D-ascorbate | 99% activity with D-ascorbate as compared to L-ascorbate | Cyanidium caldarium |  |
| 1.14.15.20 | desferrioxamine | 6fold increase of activity in the presence of 2.5 mM desferrioxamine | Cyanidium caldarium | |
| 1.14.15.20 | HClO4 | 134% activity at 5% (v/v) HClO4 | Cyanidium caldarium | |
| 1.14.15.20 | L-ascorbate | required for activity | Cyanidium caldarium | |
| 1.14.15.20 | additional information | not activated by EDTA, dehydroascorbate, and phenyledediamine | Cyanidium caldarium | |
| 1.14.15.20 | Tiron | almost as effective as desferrioxamine | Cyanidium caldarium | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.15.20 | soluble | - |
Cyanidium caldarium | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.20 | protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Cyanidium caldarium | - |
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.20 | Cyanidium caldarium | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.20 | ammonium sulfate precipitation, Blue 2-Sepharose column chromatography, DEAE column chromatography, ferredoxin-Sepharose column chromatography, and Sephadex G-75 gel filtration | Cyanidium caldarium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.20 | 0.00069 | - |
unpurified enzyme, at pH 7.6 and 42°C | Cyanidium caldarium |
| 1.14.15.20 | 0.1403 | - |
after 203fold purification, at pH 7.6 and 42°C | Cyanidium caldarium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.20 | protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Cyanidium caldarium | biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.15.20 | heme oxygenase | - |
Cyanidium caldarium |
| 1.14.15.20 | Ho1 | - |
Cyanidium caldarium |
| 1.14.15.20 | HY1 | - |
Cyanidium caldarium |
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