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Literature summary extracted from

  • Elleuche, S.; Fodor, K.; von der Heyde, A.; Klippel, B.; Wilmanns, M.; Antranikian, G.
    Group III alcohol dehydrogenase from Pectobacterium atrosepticum: insights into enzymatic activity and organization of the metal ion-containing region (2014), Appl. Microbiol. Biotechnol., 98, 4041-4051.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.2 expression in Escherichia coli Pectobacterium atrosepticum
1.1.1.71 expressed in Escherichia coli M15[pREP4] cells Pectobacterium atrosepticum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.2 D194A mutation in putative metal-coordinating residue, almost complete loss of activity Pectobacterium atrosepticum
1.1.1.2 H198A mutation in putative metal-coordinating residue, almost complete loss of activity Pectobacterium atrosepticum
1.1.1.2 H267A mutation in putative metal-coordinating residue, almost complete loss of activity Pectobacterium atrosepticum
1.1.1.2 H281A mutation in putative metal-coordinating residue, almost complete loss of activity Pectobacterium atrosepticum
1.1.1.2 H363A 107% of wild-type activity Pectobacterium atrosepticum
1.1.1.71 D194A the mutation results in substantial catalytic deficiency (2.8% compared to the wild type enzyme) Pectobacterium atrosepticum
1.1.1.71 H198A inactive Pectobacterium atrosepticum
1.1.1.71 H267A inactive Pectobacterium atrosepticum
1.1.1.71 H281A inactive Pectobacterium atrosepticum
1.1.1.71 H363A the mutant with full activity is not able to grow on butanal-containing medium Pectobacterium atrosepticum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.2 EDTA 0.1 mM, 38% residual activity. Activity is completely restored by the addition of 1 mM Co2+ and to 37% by addition of Ni2+, respectively Pectobacterium atrosepticum
1.1.1.2 NADPH strong inhibition at high NADPH concentrations, assay below 0.8 mM Pectobacterium atrosepticum
1.1.1.71 EDTA complete inhibition at 1 mM Pectobacterium atrosepticum
1.1.1.71 Fe2+ no activity at 1 mM Pectobacterium atrosepticum
1.1.1.71 Ni2+ 14% residual activity at 1 mM Pectobacterium atrosepticum
1.1.1.71 Zn2+ 14% residual activity at 1 mM Pectobacterium atrosepticum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.1
-
 NADPH pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum
1.1.1.2 0.4
-
 pentanal pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum
1.1.1.2 6.8
-
 Butanal pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.71 Co2+ required Pectobacterium atrosepticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 42000
-
-
 Pectobacterium atrosepticum
1.1.1.2 42100
-
-
 Pectobacterium atrosepticum
1.1.1.2 71000
-
 gel filtration Pectobacterium atrosepticum
1.1.1.71 42100
-
-
 Pectobacterium atrosepticum
1.1.1.71 43200
-
-
 Pectobacterium atrosepticum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Pectobacterium atrosepticum U6CL97
-
-
1.1.1.2 Pectobacterium atrosepticum DSM 18077 U6CL97
-
-
1.1.1.71 Pectobacterium atrosepticum U6CL97
-
-
1.1.1.71 Pectobacterium atrosepticum DSM18077 U6CL97
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.71 Ni-NTA column chromatography and Superdex 200 pg gel filtration Pectobacterium atrosepticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 butanal + NADPH + H+
-
 Pectobacterium atrosepticum butan-1-ol + NADP+
-
 ?
1.1.1.2 butanal + NADPH + H+
-
 Pectobacterium atrosepticum DSM18077 butan-1-ol + NADP+
-
 ?
1.1.1.2 butanal + NADPH + H+
-
 Pectobacterium atrosepticum DSM 18077 butan-1-ol + NADP+
-
 ?
1.1.1.2 additional information enzyme does not catalyze the oxidation of alcoholic substrates butanol, propanol, ethanol, methanol, glycerol, 1,3-propanediol, and 1,2-propandiol with NAD(P)+ as cofactor Pectobacterium atrosepticum ?
-
 ?
1.1.1.2 additional information enzyme does not catalyze the oxidation of alcoholic substrates butanol, propanol, ethanol, methanol, glycerol, 1,3-propanediol, and 1,2-propandiol with NAD(P)+ as cofactor Pectobacterium atrosepticum DSM18077 ?
-
 ?
1.1.1.2 additional information enzyme does not catalyze the oxidation of alcoholic substrates butanol, propanol, ethanol, methanol, glycerol, 1,3-propanediol, and 1,2-propandiol with NAD(P)+ as cofactor Pectobacterium atrosepticum DSM 18077 ?
-
 ?
1.1.1.2 pentanal + NADPH + H+
-
 Pectobacterium atrosepticum pentan-1-ol + NADP+
-
 ?
1.1.1.2 pentanal + NADPH + H+
-
 Pectobacterium atrosepticum DSM18077 pentan-1-ol + NADP+
-
 ?
1.1.1.2 pentanal + NADPH + H+
-
 Pectobacterium atrosepticum DSM 18077 pentan-1-ol + NADP+
-
 ?
1.1.1.71 butanal + NADPH + H+ 44% activity compared to pentanal Pectobacterium atrosepticum 1-butanol + NAD(P)+
-
 ?
1.1.1.71 furfural + NADPH + H+ 5% activity compared to pentanal Pectobacterium atrosepticum (furan-2-yl)methanol + NAD(P)+
-
 ?
1.1.1.71 glycoaldehyde + NADPH + H+ 5% activity compared to pentanal Pectobacterium atrosepticum ?
-
 ?
1.1.1.71 heptanal + NADPH + H+ 56% activity compared to pentanal Pectobacterium atrosepticum 1-heptanol + NAD(P)+
-
 ?
1.1.1.71 hexanal + NADPH + H+ 62% activity compared to pentanal Pectobacterium atrosepticum 1-hexanol + NAD(P)+
-
 ?
1.1.1.71 methylglyoxal + NADPH + H+ 29% activity compared to pentanal Pectobacterium atrosepticum ?
-
 ?
1.1.1.71 additional information no activity with glyoxal, methanal, and ethanal Pectobacterium atrosepticum ?
-
 ?
1.1.1.71 additional information the enzyme shows no oxidative activities towards alcoholic compounds Pectobacterium atrosepticum ?
-
 ?
1.1.1.71 additional information no activity with glyoxal, methanal, and ethanal Pectobacterium atrosepticum DSM18077 ?
-
 ?
1.1.1.71 additional information the enzyme shows no oxidative activities towards alcoholic compounds Pectobacterium atrosepticum DSM18077 ?
-
 ?
1.1.1.71 additional information no activity with glyoxal, methanal, and ethanal Pectobacterium atrosepticum DSM 18077 ?
-
 ?
1.1.1.71 additional information the enzyme shows no oxidative activities towards alcoholic compounds Pectobacterium atrosepticum DSM 18077 ?
-
 ?
1.1.1.71 octanal + NADPH + H+ 55% activity compared to pentanal Pectobacterium atrosepticum 1-octanol + NAD(P)+
-
 ?
1.1.1.71 pentanal + NADPH + H+ 100% activity Pectobacterium atrosepticum 1-pentanol + NAD(P)+
-
 ?
1.1.1.71 propanal + NADPH + H+ 14% activity compared to pentanal Pectobacterium atrosepticum 1-propanol + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.2 dimer 2 * 42100, calculated, 2 * 42000, SDS-PAGE Pectobacterium atrosepticum
1.1.1.71 ? x * 42100, calculated from amino acid sequence Pectobacterium atrosepticum
1.1.1.71 ? x * 43200, SDS-PAGE Pectobacterium atrosepticum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.2 YqhD
-
 Pectobacterium atrosepticum
1.1.1.71 ADH
-
 Pectobacterium atrosepticum
1.1.1.71 NAD(P)+-dependent alcohol dehydrogenase
-
 Pectobacterium atrosepticum
1.1.1.71 YqhD
-
 Pectobacterium atrosepticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 40
-
-
 Pectobacterium atrosepticum
1.1.1.71 40
-
-
 Pectobacterium atrosepticum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.2 20 30 30-60% of maximum activity Pectobacterium atrosepticum
1.1.1.71 10 50 30 to 60% of activity is observed between 20° and 30°C and 20% activity at 10 and 50°C, respectively Pectobacterium atrosepticum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 4 40 stable for at least 30 min Pectobacterium atrosepticum
1.1.1.2 50
-
 complete inactivation Pectobacterium atrosepticum
1.1.1.71 4 60 the enzyme is thermally stable when incubated for 30 min between 4 and 40°C. Incubation for 30 min at temperatures between 10 and 30 °C slightly increases the catalytic activity. Recombinant enzyme is completely inactivated at 50 and 60°C Pectobacterium atrosepticum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.2 20.1
-
 Butanal pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum
1.1.1.2 49.2
-
 pentanal pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 7
-
-
 Pectobacterium atrosepticum
1.1.1.71 7
-
-
 Pectobacterium atrosepticum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.71 6 8 the enzyme shows around 50% of activity at pH 6.0, pH 6.5, and pH 8.0 Pectobacterium atrosepticum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADPH
-
 Pectobacterium atrosepticum
1.1.1.71 NADPH
-
 Pectobacterium atrosepticum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.2 Pectobacterium atrosepticum calculated
-
 5.5
1.1.1.71 Pectobacterium atrosepticum calculated from amino acid sequence
-
 5.5

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.2 3
-
 Butanal pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum
1.1.1.2 123.1
-
 pentanal pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum