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Literature summary extracted from

  • Endoh, T.; Kasuga, K.; Horinouchi, M.; Yoshida, T.; Habe, H.; Nojiri, H.; Omori, T.
    Characterization and identification of genes essential for dimethyl sulfide utilization in Pseudomonas putida strain DS1 (2003), Appl. Microbiol. Biotechnol., 62, 83-91.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.34 methanesulfonate + FMNH2 + O2 Pseudomonas putida
-
 formaldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.34 methanesulfonate + FMNH2 + O2 Pseudomonas putida DS1
-
 formaldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.34 additional information Pseudomonas putida no substrates: DMSO, dimethyl sulfone ?
-
 ?
1.14.14.34 additional information Pseudomonas putida DS1 no substrates: DMSO, dimethyl sulfone ?
-
 ?
1.14.14.34 pentanesulfonate + FMNH2 + O2 Pseudomonas putida
-
 pentanal + FMN + sulfite + H2O
-
 ?
1.14.14.34 pentanesulfonate + FMNH2 + O2 Pseudomonas putida DS1
-
 pentanal + FMN + sulfite + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.34 Pseudomonas putida O85764
-
-
1.14.14.34 Pseudomonas putida DS1 O85764
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.34 82900
-
 substrate pentanesulfonate, pH 8.0, 30°C Pseudomonas putida
1.14.14.34 121000
-
 substrate methanesulfonate, pH 8.0, 30°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.34 methanesulfonate + FMNH2 + O2
-
 Pseudomonas putida formaldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.34 methanesulfonate + FMNH2 + O2
-
 Pseudomonas putida DS1 formaldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.34 additional information no substrates: DMSO, dimethyl sulfone Pseudomonas putida ?
-
 ?
1.14.14.34 additional information no substrates: DMSO, dimethyl sulfone Pseudomonas putida DS1 ?
-
 ?
1.14.14.34 pentanesulfonate + FMNH2 + O2
-
 Pseudomonas putida pentanal + FMN + sulfite + H2O
-
 ?
1.14.14.34 pentanesulfonate + FMNH2 + O2
-
 Pseudomonas putida DS1 pentanal + FMN + sulfite + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.34 SsuD
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.34 FMNH2
-
 Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.14.14.34 physiological function methanesulfonate is a metabolic intermediate of dimethyl sulfide and desulfonated by monooxygenase SsuD. SsuD is essential for desulfonation, but disruption of the SsuD gene does not affect utilization of pentanesulfonate or butanesulfonate Pseudomonas putida