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Literature summary extracted from

  • Koepke, M.; Gerth, M.L.; Maddock, D.J.; Mueller, A.P.; Liew, F.; Simpson, S.D.; Patrick, W.M.
    Reconstruction of an acetogenic 2,3-butanediol pathway involving a novel NADPH-dependent primary-secondary alcohol dehydrogenase (2014), Appl. Environ. Microbiol., 80, 3394-3403.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.4 0.6
-
 acetone pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.4 1.2
-
 Butanone pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.4 5.5
-
 acetaldehyde pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.4 65
-
 (2R,3R)-2,3-butanediol pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.4 71
-
 acetoin pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.80 0.6
-
 acetone at pH 7.5 and 25°C Clostridium autoethanogenum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Clostridium autoethanogenum F8TEL7
-
-
1.1.1.80 Clostridium autoethanogenum
-
-
-
1.1.1.80 Clostridium autoethanogenum DSM 10061
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (2R,3R)-2,3-butanediol + NADP+ no detectable activity with (2S,3S)-2,3-butanediol Clostridium autoethanogenum (R)-acetoin + NADPH + H+
-
 r
1.1.1.4 acetaldehyde + NADPH + H+
-
 Clostridium autoethanogenum ethanol + NADP+
-
 ?
1.1.1.4 acetoin + 2 NADPH + 2 H+
-
 Clostridium autoethanogenum (2R,3R)-2,3-butanediol + meso-2,3-butanediol + 2 NADP+
-
 r
1.1.1.4 acetone + NADPH + H+
-
 Clostridium autoethanogenum propan-2-ol + NADP+
-
 ?
1.1.1.4 butanone + NADPH + H+
-
 Clostridium autoethanogenum butan-2-ol + NADP+
-
 ?
1.1.1.4 additional information enzyme is a strictly NADPH-dependent primary-secondary alcohol dehydrogenase able to reduce acetoin to 2,3-butanediol. The enzyme accepts a range of 2-, 3-, and 4-carbon substrates, including the nonphysiological ketones acetone and butanone Clostridium autoethanogenum ?
-
 ?
1.1.1.80 acetone + NADPH + H+
-
 Clostridium autoethanogenum propan-2-ol + NADP+
-
 ?
1.1.1.80 acetone + NADPH + H+
-
 Clostridium autoethanogenum DSM 10061 propan-2-ol + NADP+
-
 ?
1.1.1.80 propan-2-ol + NADP+
-
 Clostridium autoethanogenum acetone + NADPH + H+
-
 ?
1.1.1.80 propan-2-ol + NADP+
-
 Clostridium autoethanogenum DSM 10061 acetone + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.80 ADH
-
 Clostridium autoethanogenum
1.1.1.80 NADPH-dependent primary-secondary alcohol dehydrogenase
-
 Clostridium autoethanogenum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.4 2.1
-
 (2R,3R)-2,3-butanediol pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.4 44
-
 Butanone pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.4 51.4
-
 acetone pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.4 93
-
 acetaldehyde pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.4 145
-
 acetoin pH 7.5, 25°C Clostridium autoethanogenum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 additional information no activity with NADH Clostridium autoethanogenum
1.1.1.4 NADPH
-
 Clostridium autoethanogenum
1.1.1.80 NADP+
-
 Clostridium autoethanogenum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.4 0.033
-
 (2R,3R)-2,3-butanediol pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.4 2
-
 acetoin pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.4 17
-
 acetaldehyde pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.4 38
-
 Butanone pH 7.5, 25°C Clostridium autoethanogenum
1.1.1.4 86
-
 acetone pH 7.5, 25°C Clostridium autoethanogenum