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Literature summary extracted from

  • Chen, M.; Narai, S.; Omura, N.; Shigi, N.; Chimnaronk, S.; Tanaka, Y.; Yao, M.
    Identification Crystallographic study of the 2-thioribothymidine-synthetic complex TtuA-TtuB from Thermus thermophilus (2016), Acta Crystallogr. Sect. F, 72, 777.781.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.15 expressed in Escherichia coli BL21 Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.1.15 vapour-diffusion method at 293 K under anaerobic conditions, 2-thioribothymidinesynthetic complex TtuA–TtuB Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor Thermus thermophilus
-
 AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor Thermus thermophilus DSM 7039
-
 AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.15 Thermus thermophilus Q72LF3
-
-
2.8.1.15 Thermus thermophilus DSM 7039 Q72LF3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor
-
 Thermus thermophilus AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?
2.8.1.15 ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor
-
 Thermus thermophilus DSM 7039 AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.1.15 TTC0106
-
 Thermus thermophilus
2.8.1.15 TtuA
-
 Thermus thermophilus