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Literature summary extracted from

  • Gohain, N.; Thomashow, L.S.; Mavrodi, D.V.; Blankenfeldt, W.
    The purification, crystallization and preliminary structural characterization of FAD-dependent monooxygenase PhzS, a phenazine-modifying enzyme from Pseudomonas aeruginosa (2006), Acta Crystallogr. Sect. F, 62, 989-992.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.218 expressed in Escherichia coli Rosetta pLysS cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.218 native and seleno-L-methionine-labelled enzyme, hanging drop vapor diffusion method, using 0.7-1.0 M lithium sulfate, 0.1 M sodium citrate pH 5.0–5.5, 0.5 M ammonium sulfate or 14-17% (w/v) PEG 10 000, 0.1 M ammonium acetate, 0.1 M Bis-Tris pH 5.5 and 23-26% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.8-6.0, 0.2 M ammonium sulfate Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.218 43600
-
-
Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.218 5-methylphenazine-1-carboxylate + NADH + O2 Pseudomonas aeruginosa
-
pyocyanin + NAD+ + CO2 + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.218 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.218 Ni-NTA affinity resin column chromatography and Superdex 200 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.218 5-methylphenazine-1-carboxylate + NADH + O2
-
Pseudomonas aeruginosa pyocyanin + NAD+ + CO2 + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.13.218 homodimer 2 * 43600, calculated from amino acid sequence Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.14.13.218 phzS
-
Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.218 FAD
-
Pseudomonas aeruginosa
1.14.13.218 NADH
-
Pseudomonas aeruginosa