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Literature summary extracted from

  • Chen, Y.; Naesvall, J.; Wu, S.; Andersson, D.I.; Selmer, M.
    Structure of AadA from Salmonella enterica: a monomeric aminoglycoside (3)(9) adenyltransferase (2015), Acta Crystallogr. Sect. D, 71, 2267-2277.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.47 to 2.5 A resolution. AadA consists of a nucleotidyltransferase domain and an alpha-helical bundle domain. AadA crystallizes as a monomer. ATP binding has to occur before binding of the aminoglycoside substrate, and ATP binding repositions the two domains for aminoglycoside binding in the interdomain cleft Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.47 D182A mutation reduces the MIC of streptomycin and spectinomycin Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.47 D182N mutation reduces the MIC of streptomycin and spectinomycin Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.47 E87A mutation reduces the MIC of streptomycin and spectinomycin to that of an AadA null strain Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.47 E87Q mutation reduces the MIC of streptomycin and spectinomycin to that of an AadA null strain Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.47 K205A mutation reduces the MIC of streptomycin and spectinomycin Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.47 R192A mutation reduces the MIC of streptomycin and spectinomycin Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.47 W112A mutation reduces the MIC of spectinomycin to that of an AadA null strain and reduces the MIC of streptomycin Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.47 W112F mutation reduces the MIC of spectinomycin to that of an AadA null strain and reduces the MIC of streptomycin Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.47 Salmonella enterica subsp. enterica serovar Typhimurium Q8ZPX9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.47 ATP + spectinomycin
-
Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + 9-adenylylspectinomycin
-
?
2.7.7.47 ATP + streptomycin
-
Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + 3''-adenylylstreptomycin
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.47 monomer small-angle X-ray scattering Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
2.7.7.47 aadA
-
Salmonella enterica subsp. enterica serovar Typhimurium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.47 Salmonella enterica subsp. enterica serovar Typhimurium calculated
-
5