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Literature summary extracted from

  • Lin, X.A.; Zhang, W.; Chen, Y.; Yao, B.; Zhang, Z.F.
    Overexpression of celB gene coding for beta-glucosidase from Pyrococcus furiosus using a baculovirus expression vector system in silkworm, Bombyx mori (2006), Z. Naturforsch. C, 61, 595-600.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.B28 synthesis expression of enzyme using a baculovirus expression vector system in silkworm, Bombyx mori and purification to about 81% homogeneity in a single heat-treatment step. The expressed beta-glucosidase accounts for more than 10% of silkworm total haemolymph proteins. The expression level reaches 10199.5 U per ml hemolymph and 19797.4 U per silkworm larva, and the specific activity of the one-step purified crude enzyme is 885 U per mg Pyrococcus furiosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B28 expression using a baculovirus expression vector system in silkworm, Bombyx mori Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B28 Pyrococcus furiosus Q51723
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B28 885
-
 recombinant protein, 90°C, pH 5.0 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B28 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + beta-D-glucopyranose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B28 105
-
 recombinant protein Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B28 5
-
 recombinant protein Pyrococcus furiosus