Any feedback?
Literature summary extracted from
- Biochemical characterization of quinolinic acid phosphoribosyltransferase from Mycobacterium tuberculosis H37Rv and inhibition of its activity by pyrazinamide (2014), PLoS ONE, 9, e100062.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.2.19 | expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 2.4.2.19 | H175A | inactive | Mycobacterium tuberculosis |
| 2.4.2.19 | K172A | inactive | Mycobacterium tuberculosis |
| 2.4.2.19 | R105A | inactive | Mycobacterium tuberculosis |
| 2.4.2.19 | R139A | inactive | Mycobacterium tuberculosis |
| 2.4.2.19 | R162A | inactive | Mycobacterium tuberculosis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.2.19 | Pyrazinamide | - |
Mycobacterium tuberculosis |  |
| 2.4.2.19 | pyrazinoic acid | - |
Mycobacterium tuberculosis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.2.19 | 0.08 | - |
quinolinic acid | at pH 7.2 and 37°C | Mycobacterium tuberculosis | |
| 2.4.2.19 | 0.39 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.2 and 37°C | Mycobacterium tuberculosis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.2.19 | Co2+ | 12% activity in the presence of Mn2+ compared to Mg2+ | Mycobacterium tuberculosis | |
| 2.4.2.19 | Fe2+ | 6% activity in the presence of Mn2+ compared to Mg2+ | Mycobacterium tuberculosis | |
| 2.4.2.19 | Mg2+ | the enzyme requires bivalent metal ions and its activity is highest in the presence of Mg2+ | Mycobacterium tuberculosis | |
| 2.4.2.19 | Mn2+ | 27% activity in the presence of Mn2+ compared to Mg2+ | Mycobacterium tuberculosis | |
| 2.4.2.19 | additional information | not stimulated by Ca2+, Zn2+, and Cu2+ | Mycobacterium tuberculosis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.19 | 58000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.2.19 | quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
nicotinic acid mononucleotide + diphosphate + CO2 | - |
? | |
| 2.4.2.19 | quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis H37Rv | - |
nicotinic acid mononucleotide + diphosphate + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.2.19 | Mycobacterium tuberculosis | P9WJJ7 | - |
- |
| 2.4.2.19 | Mycobacterium tuberculosis H37Rv | P9WJJ7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.2.19 | HisTrap column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.19 | 0.2 | - |
cell extract, at pH 7.2 and 37°C | Mycobacterium tuberculosis |
| 2.4.2.19 | 1.2 | - |
after 6.4fold purification, at pH 7.2 and 37°C | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.2.19 | quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | nicotinic acid mononucleotide + diphosphate + CO2 | - |
? | |
| 2.4.2.19 | quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | nicotinic acid mononucleotide + diphosphate + CO2 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.2.19 | NadC | - |
Mycobacterium tuberculosis |
| 2.4.2.19 | QAPRTase | - |
Mycobacterium tuberculosis |
| 2.4.2.19 | quinolinic acid phosphoribosyltransferase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.19 | 60 | - |
- |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.2.19 | 0.12 | - |
quinolinic acid | at pH 7.2 and 37°C | Mycobacterium tuberculosis | |
| 2.4.2.19 | 0.14 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.2 and 37°C | Mycobacterium tuberculosis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.19 | 9.2 | - |
- |
Mycobacterium tuberculosis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.19 | 5.2 | 11 | activity cannot be detected at pH values below 5.2 and above 11.0 | Mycobacterium tuberculosis |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 2.4.2.19 | 0.38 | - |
at pH 7.2 and 37°C | Mycobacterium tuberculosis | Pyrazinamide | |
| 2.4.2.19 | 1.37 | - |
at pH 6.2 and 37°C | Mycobacterium tuberculosis | Pyrazinamide | |
| 2.4.2.19 | 3.45 | - |
at pH 7.2 and 37°C | Mycobacterium tuberculosis | pyrazinoic acid | |
| 2.4.2.19 | 20 | - |
IC50 above 20 mM, at pH 6.2 and 37°C | Mycobacterium tuberculosis | pyrazinoic acid | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
