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Literature summary extracted from

  • Kaylor, J.J.; Radu, R.A.; Bischoff, N.; Makshanoff, J.; Hu, J.; Lloyd, M.; Eddington, S.; Bianconi, T.; Bok, D.; Travis, G.H.
    Diacylglycerol O-acyltransferase type-1 synthesizes retinyl esters in the retina and retinal pigment epithelium (2015), PLoS ONE, 10, e0125921.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 expressed in HEK-293 cells Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.20 0.0085
-
 11-cis-retinol at pH 8.0 and 37°C Mus musculus
2.3.1.20 0.0099
-
 all-trans-retinol at pH 8.0 and 37°C Mus musculus
2.3.1.20 0.0188
-
 13-cis-retinol at pH 8.0 and 37°C Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.20 45000
-
 x * 45000, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 Mueller glial cell
-
 Mus musculus
-
2.3.1.20 retina
-
 Mus musculus
-
2.3.1.20 retinal pigment epithelium cell
-
 Mus musculus
-
2.3.1.20 RPE cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 palmitoyl-CoA + 11-cis-retinol
-
 Mus musculus ?
-
 ?
2.3.1.20 palmitoyl-CoA + 13-cis-retinol
-
 Mus musculus ?
-
 ?
2.3.1.20 palmitoyl-CoA + all-trans-retinol
-
 Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.20 ? x * 45000, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 DGAT1
-
 Mus musculus
2.3.1.20 diacylglycerol O-acyltransferase type-1
-
 Mus musculus
2.3.1.20 diacylglycerol O-acyltransferase-1
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.20 malfunction enzyme-deficient mice show no effect on retinal anatomy or the ultrastructure of photoreceptor outer-segments. Enzyme loss affects retinyl-ester synthesis and total acyl-coenzyme A:retinol acyl-transferase activityin the eye Mus musculus