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Literature summary extracted from

  • Chedgy, R.J.; Koellner, T.G.; Constabel, C.P.
    Functional characterization of two acyltransferases from Populus trichocarpa capable of synthesizing benzyl benzoate and salicyl benzoate, potential intermediates in salicinoid phenolic glycoside biosynthesis (2015), Phytochemistry, 113, 149-159.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.196 expression in Escherichia coli Populus trichocarpa

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.196 H166A HTMSD motif altered to ATMSD. 97% loss of activity Populus trichocarpa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.196 0.044
-
 salicyl alcohol cosubstrate benzoyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.046
-
 benzoyl-CoA cosubstrate salicyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.052
-
 benzoyl-CoA cosubstrate benzyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.057
-
 3-Hydroxybenzyl alcohol cosubstrate benzoyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.059
-
 benzyl alcohol cosubstrate benzoyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.063
-
 benzoyl-CoA cosubstrate 3-hydroxybenzyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.067
-
 3-Hydroxybenzyl alcohol cosubstrate benzoyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.246
-
 acetyl-CoA cosubstrate salicyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.339
-
 salicyl alcohol cosubstrate acetyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.365
-
 acetyl-CoA cosubstrate benzyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.395
-
 benzyl alcohol cosubstrate acetyl-CoA, pH 7.0, 30°C Populus trichocarpa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.196 51900
-
 x * 51900, calculated and SDS-PAGE Populus trichocarpa
2.3.1.196 52600
-
 x * 52600, calculated and SDS-PAGE Populus trichocarpa

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.196 Populus trichocarpa A0A0B4VSM7
-
-
2.3.1.196 Populus trichocarpa B9I6S9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.196 leaf
-
 Populus trichocarpa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.196 acetyl-CoA + 3-hydroxybenzyl alcohol 41% of the activity with benzoyl-CoA Populus trichocarpa 3-hydroxybenzyl acetate + CoA
-
 ?
2.3.1.196 acetyl-CoA + benzyl alcohol 13% of the activity with benzoyl-CoA Populus trichocarpa benzyl acetate + CoA
-
 ?
2.3.1.196 acetyl-CoA + benzyl alcohol 51% of the activity with benzoyl-CoA Populus trichocarpa benzyl acetate + CoA
-
 ?
2.3.1.196 acetyl-CoA + salicyl alcohol 43.5% of the activity with benzoyl-CoA Populus trichocarpa salicyl acetate + CoA
-
 ?
2.3.1.196 benzoyl-CoA + 3-hydroxybenzyl alcohol 112% of the activity with benzyl alcohol Populus trichocarpa 3-hydroxybenzyl benzoate + CoA
-
 ?
2.3.1.196 benzoyl-CoA + 3-hydroxybenzyl alcohol 97% of the activity with salicyl alcohol Populus trichocarpa 3-hydroxybenzyl benzoate + CoA
-
 ?
2.3.1.196 benzoyl-CoA + benzyl alcohol
-
 Populus trichocarpa benzyl benzoate + CoA
-
 ?
2.3.1.196 benzoyl-CoA + cinnamyl alcohol 44% of the activity with salicyl alcohol Populus trichocarpa cinnamyl benzoate + CoA
-
 ?
2.3.1.196 benzoyl-CoA + coniferyl alcohol 71% of the activity with salicyl alcohol Populus trichocarpa coniferyl benzoate + CoA
-
 ?
2.3.1.196 benzoyl-CoA + salicyl alcohol
-
 Populus trichocarpa salicyl benzoate + CoA
-
 ?
2.3.1.196 cinnamoyl-CoA + 3-benzyl alcohol 28.6% of the activity with benzoyl-CoA Populus trichocarpa benzyl cinnamate + CoA
-
 ?
2.3.1.196 cinnamoyl-CoA + salicyl alcohol 11.5% of the activity with benzoyl-CoA Populus trichocarpa salicyl cinnamoate + CoA
-
 ?
2.3.1.196 additional information no substrates: cinnamoyl-CoA, coumaroyl-CoA, or caffeoyl-CoA Populus trichocarpa ?
-
 ?
2.3.1.196 additional information no substrates: coumaroyl-CoA, or caffeoyl-CoA Populus trichocarpa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.196 ? x * 51900, calculated and SDS-PAGE Populus trichocarpa
2.3.1.196 ? x * 52600, calculated and SDS-PAGE Populus trichocarpa

Synonyms

EC Number Synonyms Comment Organism
2.3.1.196 ACT47
-
 Populus trichocarpa
2.3.1.196 ACT49
-
 Populus trichocarpa
2.3.1.196 BEBT
-
 Populus trichocarpa
2.3.1.196 benzoyl-CoA: salicyl alcohol O-benzoyltransferase
-
 Populus trichocarpa
2.3.1.196 SABT
-
 Populus trichocarpa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.196 0.32
-
 acetyl-CoA cosubstrate benzyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.35
-
 salicyl alcohol cosubstrate acetyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.4
-
 benzyl alcohol cosubstrate acetyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 0.43
-
 acetyl-CoA cosubstrate salicyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 1.42
-
 3-Hydroxybenzyl alcohol cosubstrate benzoyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 1.72
-
 benzoyl-CoA cosubstrate benzyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 1.79
-
 benzyl alcohol cosubstrate benzoyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 1.97
-
 3-Hydroxybenzyl alcohol cosubstrate benzoyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 2.01
-
 benzoyl-CoA cosubstrate 3-hydroxybenzyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 2.06
-
 salicyl alcohol cosubstrate benzoyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 2.06
-
 benzoyl-CoA cosubstrate salicyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 2.25
-
 benzoyl-CoA cosubstrate 3-hydroxybenzyl alcohol, pH 7.0, 30°C Populus trichocarpa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.196 6.5 7.5
-
 Populus trichocarpa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.196 5.5 8.5 at least 50% of maximum activity Populus trichocarpa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.196 0.9
-
 acetyl-CoA cosubstrate benzyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 1
-
 benzyl alcohol cosubstrate acetyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 1
-
 salicyl alcohol cosubstrate acetyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 1.7
-
 acetyl-CoA cosubstrate salicyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 30.4
-
 benzyl alcohol cosubstrate benzoyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 30.4
-
 3-Hydroxybenzyl alcohol cosubstrate benzoyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 32.1
-
 benzoyl-CoA cosubstrate 3-hydroxybenzyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 32.6
-
 3-Hydroxybenzyl alcohol cosubstrate benzoyl-CoA, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 33.2
-
 benzoyl-CoA cosubstrate benzyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 35.5
-
 benzoyl-CoA cosubstrate 3-hydroxybenzyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 45
-
 benzoyl-CoA cosubstrate salicyl alcohol, pH 7.0, 30°C Populus trichocarpa
2.3.1.196 46.6
-
 salicyl alcohol cosubstrate benzoyl-CoA, pH 7.0, 30°C Populus trichocarpa