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Literature summary extracted from

  • Schmidtmann, E.; Koenig, A.C.; Orwat, A.; Leister, D.; Hartl, M.; Finkemeier, I.
    Redox regulation of Arabidopsis mitochondrial citrate synthase (2014), Mol. Plant, 7, 156-169.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.3.16 thioredoxin a strong and more than 8fold activation of enzyme activity is observed for isoform CS4 with Escherichia coli thioredoxin coupled to reduction by NTR and NADPH Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.16 expressed in Escherichia coli BL21 cells Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 H2O2 about 54% inhibition at 0.4 mM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 71.6
-
 acetyl-CoA at pH 8.0 and 20°C Arabidopsis thaliana
2.3.3.16 90.3
-
 oxaloacetate at pH 8.0 and 20°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.16 mitochondrion
-
 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 100000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.16 acetyl-CoA + H2O + oxaloacetate Arabidopsis thaliana
-
 citrate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Arabidopsis thaliana P20115
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16 Ni-NTA column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + H2O + oxaloacetate
-
 Arabidopsis thaliana citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 dimer or oligomer the CS4 isoform is not only present as dimer but also as high-molecular-weight oligomer under native conditions Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.3.3.16 CS4 isoform Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 20
-
-
 Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.3.16 35
-
 nearly 50% of the activity is retained at 35°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 8
-
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.3.16 5 9 nearly 50% of the activity is retained at pH 5.0 or 9.0 Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.3.16 1827
-
 oxaloacetate at pH 8.0 and 20°C Arabidopsis thaliana
2.3.3.16 4339
-
 acetyl-CoA at pH 8.0 and 20°C Arabidopsis thaliana