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Literature summary extracted from

  • Jiang, W.; Xia, B.; Liu, Z.
    A serine hydroxymethyltransferase from marine bacterium Shewanella algae: Isolation, purification, characterization and l-serine production (2013), Microbiol. Res., 168, 477-484.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.2.1 CO(NH2)2 activates Shewanella algae
2.1.2.1 EDTA activates Shewanella algae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 gene glyA, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) Shewanella algae

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.1 additional information the SaSHMT has the potential for industrial applications due to its tolerance of alkaline environment and a relatively high enzymatic conversion rate Shewanella algae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 Co2+ slight inhibition; strong inhibition Shewanella algae
2.1.2.1 CTAB strong inhibition Shewanella algae
2.1.2.1 DTT strong inhibition Shewanella algae
2.1.2.1 Fe2+ slight inhibition Shewanella algae
2.1.2.1 Hg2+ strong inhibition Shewanella algae
2.1.2.1 SDS strong inhibition Shewanella algae
2.1.2.1 Zn2+ strong inhibition Shewanella algae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.1 Ba2+ activates Shewanella algae
2.1.2.1 Ca2+ activates Shewanella algae
2.1.2.1 K+ activates Shewanella algae
2.1.2.1 Mg2+ activates Shewanella algae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 45300
-
 x * 45300, recombinant GST-tagged enzyme, SDS-PAGE Shewanella algae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Shewanella algae
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 additional information Shewanella algae the enzyme shows high serine hydroxymethyltransferase activity and L-serine production ?
-
 ?
2.1.2.1 tetrahydrofolate + L-serine Shewanella algae
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Shewanella algae R9YJZ9 gene glyA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 recombinant GST-tagged enzyme from Escherichia coli strain Bl21(DE3) by glutathione affinity chromatography Shewanella algae

Storage Stability

EC Number Storage Stability Organism
2.1.2.1 4°C, purified recombinant enzyme, 96 h, over 60% of maximal activity remains Shewanella algae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Shewanella algae tetrahydrofolate + L-serine
-
 r
2.1.2.1 additional information the enzyme shows high serine hydroxymethyltransferase activity and L-serine production Shewanella algae ?
-
 ?
2.1.2.1 tetrahydrofolate + L-serine
-
 Shewanella algae 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 ? x * 45300, recombinant GST-tagged enzyme, SDS-PAGE Shewanella algae

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Shewanella algae
2.1.2.1 SHMT
-
 Shewanella algae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.1 34
-
-
 Shewanella algae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.2.1 40
-
 the enzyme retains over 45% of its maximal activity after incubation at 40°C for 3 h Shewanella algae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 7
-
-
 Shewanella algae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.1 5.5 9.5 maximal activity at pH 7.0, 88% of the maximal activity at pH 7.0-8.0, less than 20% of maximal activity at pH 9.5, nearly no activity below pH 5.5 Shewanella algae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.2.1 5.5 9 purified recombinant SaSHMT shows significant stability under weakly acidic and alkaline environment at pH 6.5-9.0. Without any stabilizer at pH 6.5-9.0 for 96 h at 4°C over 80% of maximum activity is retained, at pH 5.5-6.0 less than 80% activity remains, the enzyme is unstable below pH 5.5 Shewanella algae

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 5,10-methylenetetrahydrofolate
-
 Shewanella algae
2.1.2.1 pyridoxal 5'-phosphate
-
 Shewanella algae
2.1.2.1 tetrahydrofolate
-
 Shewanella algae