Literature summary extracted from

Deptula, P.; Kylli, P.; Chamlagain, B.; Holm, L.; Kostiainen, R.; Piironen, V.; Savijoki, K.; Varmanen, P.
BluB/CobT2 fusion enzyme activity reveals mechanisms responsible for production of active form of vitamin B12 by Propionibacterium freudenreichii (2015), Microb. Cell Fact., 14, 186.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.4.2.21	expressed as fusion protein BluB/CobT2 in Escherichia coli KRX cells	Propionibacterium freudenreichii

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.4.2.21	66400	-	x * 66400, fusion protein BluB/CobT2, SDS-PAGE	Propionibacterium freudenreichii

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.4.2.21	beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole	Propionibacterium freudenreichii	-	nicotinate + alpha-ribazole 5'-phosphate	-	?
2.4.2.21	beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole	Propionibacterium freudenreichii DSM 4902	-	nicotinate + alpha-ribazole 5'-phosphate	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.2.21	Propionibacterium freudenreichii	D7GJ95	Propionibacterium freudenreichii subsp. shermanii	-
2.4.2.21	Propionibacterium freudenreichii DSM 4902	D7GJ95	Propionibacterium freudenreichii subsp. shermanii	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.2.21	beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole	-	Propionibacterium freudenreichii	nicotinate + alpha-ribazole 5'-phosphate	-	?
2.4.2.21	beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole	-	Propionibacterium freudenreichii DSM 4902	nicotinate + alpha-ribazole 5'-phosphate	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.4.2.21	?	x * 66400, fusion protein BluB/CobT2, SDS-PAGE	Propionibacterium freudenreichii

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.2.21	CobT2	-	Propionibacterium freudenreichii

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Release 2023.1 (January 2023)