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Literature summary extracted from

  • Eudes, A.; Juminaga, D.; Baidoo, E.E.; Collins, F.W.; Keasling, J.D.; Loque, D.
    Production of hydroxycinnamoyl anthranilates from glucose in Escherichia coli (2013), Microb. Cell Fact., 12, 62.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.144 synthesis coexpression of HCBT and 4-coumarate:CoA 4CL1 ligase from Nicotiana tabacum in the Escherichia coli anthranilate-accumulating strain W3110 trpD9923 allows the production of Avn D [N-(4'-hydroxycinnamoyl)-anthranilic acid] and Avn F [N-(3',4'-dihydroxycinnamoyl)-anthranilic acid] upon feeding with p-coumarate and caffeate, respectively. Additional expression of a tyrosine ammonia-lyase from Rhodotorula glutinis (TAL) leads to the conversion of endogenous tyrosine into p-coumarate and results in the production of Avn D [N-(4'-hydroxycinnamoyl)-anthranilic acid] from glucose. A 135fold improvement in Avn D titer is achieved by boosting tyrosine production. Expression of either the p-coumarate 3-hydroxylase Sam5 from Saccharothrix espanensis or the hydroxylase complex HpaBC from Escherichia coli results in the endogenous production of caffeate and biosynthesis of Avn F [N-(3',4'-dihydroxycinnamoyl)-anthranilic acid] Dianthus caryophyllus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.144 Dianthus caryophyllus O24645 isoform HCBT1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.144 4-coumaroyl-CoA + anthranilate
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 Dianthus caryophyllus CoA + N-4-(coumaroyl)anthranilate
-
 ?
2.3.1.144 benzoyl-CoA + anthranilate
-
 Dianthus caryophyllus CoA + N-benzoylanthranilate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.144 HCBT
-
 Dianthus caryophyllus
2.3.1.144 hydroxycinnamoyl/benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase
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 Dianthus caryophyllus