Literature summary extracted from

Lin, P.; Mi, L.; Morioka, A.; Yoshino, K.; Konishi, S.; Xu, S.; Papanek, B.; Riley, L.; Guss, A.; Liao, J.
Consolidated bioprocessing of cellulose to isobutanol using Clostridium thermocellum (2015), Metab. Eng., 31, 44-52.

Application

EC Number	Application	Comment	Organism
1.2.7.7	synthesis	engineering of Clostridium thermocellum to produce isobutanol. Both the native 2-oxoisovalerate-oxidoreductase KOR, EC 1.2.7.7, and the heterologous Lactococcus lactis 2-oxoisovalerate decarboxylase KIVD, EC 4.1.1.74, expressed are responsible for isobutanol production.The plasmid is integrated into the chromosome by single crossover. The resulting strain is stable without antibiotic selection pressure and produces 5.4g/l of isobutanol from cellulose in minimal medium at 50°C within 75 h, corresponding to 41% of theoretical yield	Acetivibrio thermocellus
4.1.1.74	synthesis	engineering of Clostridium thermocellum to produce isobutanol. Both the native 2-oxoisovalerate-oxidoreductase KOR, EC 1.2.7.7, and the heterologous Lactococcus lactis 2-oxoisovalerate decarboxylase KIVD, EC 4.1.1.74, expressed are responsible for isobutanol production.The plasmid is integrated into the chromosome by single crossover. The resulting strain is stable without antibiotic selection pressure and produces 5.4g/l of isobutanol from cellulose in minimal medium at 50°C within 75 h, corresponding to 41% of theoretical yield	Lactococcus lactis

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.2.7.7	Acetivibrio thermocellus	-	-	-
1.2.7.7	Acetivibrio thermocellus DSM 1313	-	-	-
4.1.1.74	Lactococcus lactis	-	-	-

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Release 2023.1 (January 2023)