EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.1.7 | synthesis | sucrose phosphorylase is a promising biocatalyst for the production of special sugars and glycoconjugates, but its transglycosylation activity rarely exceeds the competing hydrolytic reaction | Bifidobacterium adolescentis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.7 | D342A | saturation mutagensis, transglucosylation and hydrolytic side activity of the mutant compared to the wild-type | Bifidobacterium adolescentis |
2.4.1.7 | H234A | saturation mutagensis, transglucosylation and hydrolytic side activity of the mutant compared to the wild-type | Bifidobacterium adolescentis |
2.4.1.7 | L343A | saturation mutagensis, transglucosylation and hydrolytic side activity of the mutant compared to the wild-type | Bifidobacterium adolescentis |
2.4.1.7 | P134A | saturation mutagensis, transglucosylation and hydrolytic side activity of the mutant compared to the wild-type | Bifidobacterium adolescentis |
2.4.1.7 | Q345A | saturation mutagensis, transglucosylation and hydrolytic side activity of the mutant compared to the wild-type | Bifidobacterium adolescentis |
2.4.1.7 | R135A | saturation mutagensis, transglucosylation and hydrolytic side activity of the mutant compared to the wild-type | Bifidobacterium adolescentis |
2.4.1.7 | Y132A | saturation mutagensis, transglucosylation and hydrolytic side activity of the mutant compared to the wild-type | Bifidobacterium adolescentis |
2.4.1.7 | Y196A | saturation mutagensis, transglucosylation and hydrolytic side activity of the mutant compared to the wild-type | Bifidobacterium adolescentis |
2.4.1.7 | Y344A | saturation mutagensis, transglucosylation and hydrolytic side activity of the mutant compared to the wild-type | Bifidobacterium adolescentis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.7 | sucrose + phosphate | Bifidobacterium adolescentis | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.7 | Bifidobacterium adolescentis | Q84HQ2 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.7 | sucrose + D-arabitol | - |
Bifidobacterium adolescentis | pyridoxine + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.7 | sucrose + phosphate | - |
Bifidobacterium adolescentis | D-fructose + alpha-D-glucose 1-phosphate | - |
r |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.1.7 | additional information | mapping of the acceptor site of the enzyme by saturation mutagenesis and screening, overview. Residues Arg135, Leu343, and Tyr344 contribute to the specificity for phosphate, residues Tyr132 and Asp342 contribute to the specificity for D-fructose, and residues Pro134, Tyr196, His234, Gln345 contribute to the specificity for both. Alternative acceptors that are glycosylated rather efficiently (e.g. D-arabitol) interact with the same residues as fructose, whereas poor acceptors like pyridoxine do not seem to make any specific interactions with the enzyme | Bifidobacterium adolescentis |