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Literature summary extracted from

  • Wu, G.; Yuan, M.; Wei, L.; Zhang, Y.; Lin, Y.; Zhang, L.; Liu, Z.
    Characterization of a novel cold-adapted phosphinothricin N-acetyltransferase from the marine bacterium Rhodococcus sp. strain YM12 (2014), J. Mol. Catal. B, 104, 23-28.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.183 expression in Escherichia coli Rhodococcus sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.183 molecular modeling of structure. Enzyme is a dimer. Ring stacking and a salt bridge derived from residues His114-Trp152 and Arg72-Glu82, respectively, are important forces for the subunit interaction Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.183 0.076
-
L-phosphinothricin pH 8.0, 35°C Rhodococcus sp.
2.3.1.183 0.156
-
L-methionine sulfoximine pH 8.0, 35°C Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.183 44000
-
x * 44000, recombinant GST-fusion protein Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.183 Rhodococcus sp. M1EYW6
-
-
2.3.1.183 Rhodococcus sp. YM12 M1EYW6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.183 acetyl-CoA + L-methionine sulfoximine
-
Rhodococcus sp. ?
-
?
2.3.1.183 acetyl-CoA + L-methionine sulfoximine
-
Rhodococcus sp. YM12 ?
-
?
2.3.1.183 acetyl-CoA + L-phosphinothricin
-
Rhodococcus sp. CoA + L-N-acetylphosphinothricin
-
?
2.3.1.183 acetyl-CoA + L-phosphinothricin
-
Rhodococcus sp. YM12 CoA + L-N-acetylphosphinothricin
-
?
2.3.1.183 additional information no substrate: glyphosate Rhodococcus sp. ?
-
?
2.3.1.183 additional information no substrate: glyphosate Rhodococcus sp. YM12 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.183 ? x * 44000, recombinant GST-fusion protein Rhodococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.183 35
-
-
Rhodococcus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.183
-
10 more than 50% of maximum activity Rhodococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.183 30
-
80 min, more than 80% residual activity Rhodococcus sp.
2.3.1.183 50
-
100 min, 60% residual activity Rhodococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.183 0.5
-
L-methionine sulfoximine pH 8.0, 35°C Rhodococcus sp.
2.3.1.183 2.18
-
L-phosphinothricin pH 8.0, 35°C Rhodococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.183 8
-
-
Rhodococcus sp.