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Literature summary extracted from

  • Li, L.; Liu, X.; Yang, W.; Xu, F.; Wang, W.; Feng, L.; Bartlam, M.; Wang, L.; Rao, Z.
    Crystal structure of long-chain alkane monooxygenase (LadA) in complex with coenzyme FMN: unveiling the long-chain alkane hydroxylase (2008), J. Mol. Biol., 376, 453-465.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.28 expression in Escherichia coli Geobacillus thermodenitrificans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.14.28 crystals of LadA and the LadA:FMN complex are grown using the hanging-drop vapor-diffusion method at 18°C, crystal structure of the enzyme in the apoenzyme form and its complex with FMNH2 Geobacillus thermodenitrificans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.28 C14A point mutation completely abolishes the catalytic activity Geobacillus thermodenitrificans
1.14.14.28 H17F the mutant maintains the same dimeric form as the wild type, point mutation completely abolishes the catalytic activity Geobacillus thermodenitrificans
1.14.14.28 H311F the mutant maintains the same dimeric form as the wild type, point mutation completely abolishes the catalytic activity Geobacillus thermodenitrificans
1.14.14.28 Q79L the mutant maintains the same dimeric form as the wild type, point mutation completely abolishes the catalytic activity Geobacillus thermodenitrificans
1.14.14.28 T63F the mutant maintains the same dimeric form as the wild type, point mutation completely abolishes the catalytic activity Geobacillus thermodenitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.28 50466
-
 2 * 50466, calculated from sequence Geobacillus thermodenitrificans
1.14.14.28 105000
-
 gel filtration Geobacillus thermodenitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.28 a long-chain alkane + FMNH2 + O2 Geobacillus thermodenitrificans
-
 a long-chain primary alcohol + FMN + H2O
-
 ?
1.14.14.28 a long-chain alkane + FMNH2 + O2 Geobacillus thermodenitrificans NG80-2
-
 a long-chain primary alcohol + FMN + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.28 Geobacillus thermodenitrificans A4IU28
-
-
1.14.14.28 Geobacillus thermodenitrificans NG80-2 A4IU28
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.28
-
 Geobacillus thermodenitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.28 a long-chain alkane + FMNH2 + O2
-
 Geobacillus thermodenitrificans a long-chain primary alcohol + FMN + H2O
-
 ?
1.14.14.28 a long-chain alkane + FMNH2 + O2 the enzyme is capable of converting alkanes ranging from C15 to C36 into their corresponding primary alcohols Geobacillus thermodenitrificans a long-chain primary alcohol + FMN + H2O
-
 ?
1.14.14.28 a long-chain alkane + FMNH2 + O2
-
 Geobacillus thermodenitrificans NG80-2 a long-chain primary alcohol + FMN + H2O
-
 ?
1.14.14.28 a long-chain alkane + FMNH2 + O2 the enzyme is capable of converting alkanes ranging from C15 to C36 into their corresponding primary alcohols Geobacillus thermodenitrificans NG80-2 a long-chain primary alcohol + FMN + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.28 homodimer 2 * 50466, calculated from sequence Geobacillus thermodenitrificans

Synonyms

EC Number Synonyms Comment Organism
1.14.14.28 LADA
-
 Geobacillus thermodenitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.28 7.5
-
 assay at Geobacillus thermodenitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.28 FMNH2
-
 Geobacillus thermodenitrificans