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Literature summary extracted from

  • Cui, L.; Yao, S.; Dai, X.; Yin, Q.; Liu, Y.; Jiang, X.; Wu, Y.; Qian, Y.; Pang, Y.; Gao, L.; Xia, T.
    Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis) (2016), J. Exp. Bot., 67, 2285-2297.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.91 gene CsUGT78A14, DNA and amino acid sequence determination and analysis and sequence comparisons of the enzymes from different cultivars, phylogenetic analysis, expression profile of gene CsUGT78A14 is correlated with the accumulation patterns of F-glycosides, qRT-PCR, transcriptome sequencing of Camellia sinensis var. sinensis cvs. Nongkangzao, Huangjinya, and Quntizhong, overview Camellia sinensis
2.4.1.91 gene CsUGT78A14, DNA and amino acid sequence determination and analysis, genome and transcriptome analysis and sequence comparisons of the enzymes from different cultivars, phylogenetic analysis, expression profile, qRT-PCR, transcriptome sequencing of Camellia sinensis var. sinensis cvs. Nongkangzao, Huangjinya, and Quntizhong, overview Camellia sinensis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.91 Q373H site-directed mutagenesis Camellia sinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.91 additional information
-
 additional information Michaelis-Menten kinetics Camellia sinensis
2.4.1.91 0.0119
-
 quercetin pH 7.5, 30°C Camellia sinensis
2.4.1.91 0.0141
-
 UDP-glucose pH 7.5, 30°C Camellia sinensis
2.4.1.91 0.0238
-
 myricetin pH 7.5, 30°C Camellia sinensis
2.4.1.91 0.0309
-
 kaempferol pH 7.5, 30°C Camellia sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.91 UDP-glucose + a flavonol Camellia sinensis
-
 UDP + a flavonol 3-O-beta-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.91 Camellia sinensis A0A109P632 var. assamica, gene CsUGT78A14
-
2.4.1.91 Camellia sinensis A0A109P632 var. sinensis, cv. Nongkangzao, Huangjinya, and Quntizhong, gene CsUGT78A14
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.91 additional information enzyme expression profile, transcriptome analysis, the enzyme expression is correlated with the accumulation patterns of beta-glucogallin and the glycosylated flavonols, qRT-PCR Camellia sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.91 additional information product identification by mass spectrometry, quantification by HPLC. Sugar receptor and sugar donor specificity of enzyme CsUGT78A14, homology modelling analysis Camellia sinensis ?
-
 ?
2.4.1.91 UDP-glucose + a flavonol
-
 Camellia sinensis UDP + a flavonol 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-glucose + apigenin
-
 Camellia sinensis UDP + apigenin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-glucose + cyanidin
-
 Camellia sinensis UDP + cyanidin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-glucose + epicatechin
-
 Camellia sinensis UDP + epicatechin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-glucose + eriodictyol
-
 Camellia sinensis UDP + eriodictyol 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-glucose + kaempferol
-
 Camellia sinensis UDP + kaempferol 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-glucose + myricetin
-
 Camellia sinensis UDP + myricetin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-glucose + naringenin
-
 Camellia sinensis UDP + ?
-
 ?
2.4.1.91 UDP-glucose + quercetin
-
 Camellia sinensis UDP + quercetin 3-O-beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.91 3GlcT
-
 Camellia sinensis
2.4.1.91 CsUGT78A1
-
 Camellia sinensis
2.4.1.91 flavonoid 3-O-glycosyltransferase
-
 Camellia sinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.91 30
-
 assay at Camellia sinensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.91 3.11
-
 myricetin pH 7.5, 30°C Camellia sinensis
2.4.1.91 3.32
-
 quercetin pH 7.5, 30°C Camellia sinensis
2.4.1.91 3.61
-
 UDP-glucose pH 7.5, 30°C Camellia sinensis
2.4.1.91 6.75
-
 kaempferol pH 7.5, 30°C Camellia sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.91 7.5
-
 assay at Camellia sinensis

General Information

EC Number General Information Comment Organism
2.4.1.91 evolution gene CsUGT78A14 and CsUGT78A15 share relatively higher identity with several flavonoid-3-O-glucosyltransferases, including VvGT1, VvGT6, AcF3GT1, and AcGaT, homology modelling analysis Camellia sinensis
2.4.1.91 metabolism the expression profiles of the genes CsUGT84A22, CsUGT78A14, and CsUGT78A15 are correlated with the accumulation patterns of beta-glucogallin and the glycosylated flavonols which indicates that the three CsUGT genes are involved in the biosynthesis of astringent compounds in Camellia sinensis Camellia sinensis
2.4.1.91 additional information the Gln373 residue plays a catalytically crucial role for flavonoid 3-O-glucosyltransferase activity. Enzyme expression profile, the enzyme expression is correlated with the accumulation patterns of beta-glucogallin and the glycosylated flavonols. Sequence comparison of UDP-glucosyltransferase CsUGT78A14 and UDP-galactosyltransferase CsUGT78A15 Camellia sinensis
2.4.1.91 physiological function the enzyme is responsible for the biosynthesis of flavonol 3-O-monoglycoside, the biosynthesis of astringent flavonol 3-O-monoglycoside compounds in tea plants Camellia sinensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.91 130.7
-
 myricetin pH 7.5, 30°C Camellia sinensis
2.4.1.91 218.4
-
 kaempferol pH 7.5, 30°C Camellia sinensis
2.4.1.91 256
-
 UDP-glucose pH 7.5, 30°C Camellia sinensis
2.4.1.91 279
-
 quercetin pH 7.5, 30°C Camellia sinensis