BRENDA - Enzyme Database

Substrate and phospholipid specificity of the purified mannitol permease of Escherichia coli

Jacobson, G.; Tanney, L.; Kelly, D.; Palman, K.; Corn, S.; J. Cell. Biochem. 23, 231-240 (1983)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.197
cardiolipin
-
Escherichia coli
2.7.1.197
phosphatidylethanolamine
-
Escherichia coli
2.7.1.197
phosphatidylglycerol
-
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.197
2-amino-2-deoxy-D-mannitol
63% residual activity at 2 mM
Escherichia coli
2.7.1.197
2-deoxy-D-mannitol
73% residual activity at 2 mM; 74% residual activity at 2 mM
Escherichia coli
2.7.1.197
D-arabitol
-
Escherichia coli
2.7.1.197
D-glucitol
73% residual activity at 2 mM
Escherichia coli
2.7.1.197
D-mannoheptitol
9% residual activity at 2 mM
Escherichia coli
2.7.1.197
D-mannonic acid
-
Escherichia coli
2.7.1.197
additional information
1,6-dibromo-1,6-dideoxy-D-mannitol, D,L-threitol, L-threitol, erythritol, and glycerol do not inhibit the enzyme
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.197
0.01
-
D-mannitol[side 1]
at pH 8.0 and 37C
Escherichia coli
2.7.1.197
0.2
-
2-amino-2-deoxy-D-mannitol[side 1]
at pH 8.0 and 37C
Escherichia coli
2.7.1.197
0.5
-
2-deoxy-D-mannitol[side 1]
at pH 8.0 and 37C
Escherichia coli
2.7.1.197
0.5
-
D-arabitol[side 1]
Km above 0.5 mM, at pH 8.0 and 37C
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.197
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1]
Escherichia coli
the enzyme is highly, but not absolutely, specific for D-mannitol
[protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.197
Escherichia coli
P00550
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.197
additional information
no activity with D-galactitol, D-ribitol, and D-xylitol
736548
Escherichia coli
?
-
-
-
-
2.7.1.197
[protein]-Npi-phospho-L-histidine + 2-amino-2-deoxy-D-mannitol[side 1]
50% activity compared to D-mannitol
736548
Escherichia coli
[protein]-L-histidine + 2-amino-2-deoxy-D-mannitol 1-phosphate[side 2]
-
-
-
?
2.7.1.197
[protein]-Npi-phospho-L-histidine + 2-deoxy-D-mannitol[side 1]
32% activity compared to D-mannitol
736548
Escherichia coli
[protein]-L-histidine + 2-deoxy-D-mannitol 1-phosphate[side 2]
-
-
-
?
2.7.1.197
[protein]-Npi-phospho-L-histidine + D-arabitol[side 1]
11% activity compared to D-mannitol
736548
Escherichia coli
[protein]-L-histidine + D-arabitol 1-phosphate[side 2]
-
-
-
?
2.7.1.197
[protein]-Npi-phospho-L-histidine + D-glucitol[side 1]
37% activity compared to D-mannitol
736548
Escherichia coli
[protein]-L-histidine + D-glucitol 1-phosphate[side 2]
-
-
-
?
2.7.1.197
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1]
the enzyme is highly, but not absolutely, specific for D-mannitol
736548
Escherichia coli
[protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.1.197
37
-
assay at
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.197
8
-
assay at
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.197
cardiolipin
-
Escherichia coli
2.7.1.197
phosphatidylethanolamine
-
Escherichia coli
2.7.1.197
phosphatidylglycerol
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.197
2-amino-2-deoxy-D-mannitol
63% residual activity at 2 mM
Escherichia coli
2.7.1.197
2-deoxy-D-mannitol
73% residual activity at 2 mM; 74% residual activity at 2 mM
Escherichia coli
2.7.1.197
D-arabitol
-
Escherichia coli
2.7.1.197
D-glucitol
73% residual activity at 2 mM
Escherichia coli
2.7.1.197
D-mannoheptitol
9% residual activity at 2 mM
Escherichia coli
2.7.1.197
D-mannonic acid
-
Escherichia coli
2.7.1.197
additional information
1,6-dibromo-1,6-dideoxy-D-mannitol, D,L-threitol, L-threitol, erythritol, and glycerol do not inhibit the enzyme
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.197
0.01
-
D-mannitol[side 1]
at pH 8.0 and 37C
Escherichia coli
2.7.1.197
0.2
-
2-amino-2-deoxy-D-mannitol[side 1]
at pH 8.0 and 37C
Escherichia coli
2.7.1.197
0.5
-
2-deoxy-D-mannitol[side 1]
at pH 8.0 and 37C
Escherichia coli
2.7.1.197
0.5
-
D-arabitol[side 1]
Km above 0.5 mM, at pH 8.0 and 37C
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.197
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1]
Escherichia coli
the enzyme is highly, but not absolutely, specific for D-mannitol
[protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.197
additional information
no activity with D-galactitol, D-ribitol, and D-xylitol
736548
Escherichia coli
?
-
-
-
-
2.7.1.197
[protein]-Npi-phospho-L-histidine + 2-amino-2-deoxy-D-mannitol[side 1]
50% activity compared to D-mannitol
736548
Escherichia coli
[protein]-L-histidine + 2-amino-2-deoxy-D-mannitol 1-phosphate[side 2]
-
-
-
?
2.7.1.197
[protein]-Npi-phospho-L-histidine + 2-deoxy-D-mannitol[side 1]
32% activity compared to D-mannitol
736548
Escherichia coli
[protein]-L-histidine + 2-deoxy-D-mannitol 1-phosphate[side 2]
-
-
-
?
2.7.1.197
[protein]-Npi-phospho-L-histidine + D-arabitol[side 1]
11% activity compared to D-mannitol
736548
Escherichia coli
[protein]-L-histidine + D-arabitol 1-phosphate[side 2]
-
-
-
?
2.7.1.197
[protein]-Npi-phospho-L-histidine + D-glucitol[side 1]
37% activity compared to D-mannitol
736548
Escherichia coli
[protein]-L-histidine + D-glucitol 1-phosphate[side 2]
-
-
-
?
2.7.1.197
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1]
the enzyme is highly, but not absolutely, specific for D-mannitol
736548
Escherichia coli
[protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.1.197
37
-
assay at
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.197
8
-
assay at
Escherichia coli