Literature summary extracted from

Mayer, J.; Kranz, B.; Fischer, L.
Continuous production of lactulose by immobilized thermostable beta-glycosidase from Pyrococcus furiosus (2010), J. Biotechnol., 145, 387-393.

Application

EC Number	Application	Comment	Organism
3.2.1.B28	synthesis	continuous enzymatic process for the production of the prebiotic disaccharide lactulose through transgalactosylation by CelB. CelB is immobilized onto anion-exchange resin Amberlite IRA-93 or onto Eupergit C with immobilization yields of 72% and 83%, respectively, giving specific activities of 920 nkat/g dry carrier and 1500 nkat/g dry carrier at 75°C with 4-nitrophenyl-beta-D-galactopyranoside as substrate. Maximum lactulose yields of 43% related to the initial lactose concentration are reached. The corresponding productivities are 52 g lactulose per l and h using Amberlite IRA-93 and 15 g lactulose per l and h unsing Eupergit C, respectively. While both carrier-bound CelB preparations are 100% stable for at least 14 days, the half-life of the free CelB in the enzyme membrane reactor is only about 1.5 days	Pyrococcus furiosus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.B28	Pyrococcus furiosus	Q51723	-	-

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Release 2023.1 (January 2023)