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Literature summary extracted from

  • Ohshiro, T.; Ishii, Y.; Matsubara, T.; Ueda, K.; Izumi, Y.; Kino, K.; Kirimura, K.
    Dibenzothiophene desulfurizing enzymes from moderately thermophilic bacterium Bacillus subtilis WU-S2B: purification, characterization and overexpression (2005), J. Biosci. Bioeng., 100, 266-273.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.21 gene bdsC, recombinant overexpression in Escherichia coli Bacillus subtilis
1.14.14.22 gene bdsA, recombinant overexpression in Escherichia coli, coexpression with chaperone groEL/groES genes Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.21 1,10-phenanthroline
-
 Bacillus subtilis
1.14.14.21 2,2'-dithiobis(5-nitropyridine)
-
 Bacillus subtilis
1.14.14.21 4-chloromercuribenzoate
-
 Bacillus subtilis
1.14.14.21 8-Quinolinol
-
 Bacillus subtilis
1.14.14.21 Ag+
-
 Bacillus subtilis
1.14.14.21 Cu2+
-
 Bacillus subtilis
1.14.14.21 diethyldithiocarbamate
-
 Bacillus subtilis
1.14.14.21 Hg2+
-
 Bacillus subtilis
1.14.14.22 1,10-phenanthroline
-
 Bacillus subtilis
1.14.14.22 4-chloromercuribenzoate
-
 Bacillus subtilis
1.14.14.22 Ag+
-
 Bacillus subtilis
1.14.14.22 biphenyl
-
 Bacillus subtilis
1.14.14.22 Co2+
-
 Bacillus subtilis
1.14.14.22 Cu2+
-
 Bacillus subtilis
1.14.14.22 EDTA
-
 Bacillus subtilis
1.14.14.22 Hg2+
-
 Bacillus subtilis
1.14.14.22 Ni2+
-
 Bacillus subtilis
1.14.14.22 Zn2+
-
 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.21 45000
-
 4 * 45000, SDS-PAGE Bacillus subtilis
1.14.14.21 200000
-
 gel filtration Bacillus subtilis
1.14.14.22 45000
-
 4 * 45000, SDS-PAGE Bacillus subtilis
1.14.14.22 174000
-
 recombinant enzyme, gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2 Bacillus subtilis
-
 dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2 Bacillus subtilis WU-S2B
-
 dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Bacillus subtilis
-
 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.21 Bacillus subtilis
-
 gene bdsC
-
1.14.14.21 Bacillus subtilis WU-S2B
-
 gene bdsC
-
1.14.14.22 Bacillus subtilis
-
 gene bdsA
-
1.14.14.22 Bacillus subtilis WU-S2B
-
 gene bdsA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.21 native enzyme 19.2fold from strain WU-S2B by two different steps of anion exchange chromatography and one step of hydrophobic interaction chromatography, recombinant enzyme from Escherichia coli 4.5fold to homogeneity by anion exchange and hydrophobic interaction chromatography Bacillus subtilis
1.14.14.22 native enzyme 82.6fold from strain WU-S2B by three different steps of anion exchange chromatography and one step of hydrophobic interaction chromatography, gel filtration, and another step of anion exchange chromatography, recombinant enzyme from Escherichia coli 17.0fold to homogeneity by anion exchange and hydrophobic interaction chromatography, and again anion exchange chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.21 84.2
-
 purified native enzyme, pH 7.0, 50°C Bacillus subtilis
1.14.14.21 163.2
-
 purified recombinant enzyme, pH 7.0, 50°C Bacillus subtilis
1.14.14.22 855
-
 purified native enzyme, pH 7.0, 50°C Bacillus subtilis
1.14.14.22 875
-
 purified recombinant enzyme, pH 7.0, 50°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.21 1-methyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis 1-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis WU-S2B 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 2-methyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis 2-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 3,4-benzo-dibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis 3,4-benzo-dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 3-methyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis 3-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 4,6-diethyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis 4,6-diethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis WU-S2B 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 4-methyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 4-methyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis WU-S2B 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis WU-S2B dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 additional information coupled reaction with flavin reductase (TdsD) from thermophilic Paenibacillus sp. strain A11-2. Substrate specificity, overview. No activity with 4,6-di-n-propyl-DBT, 4,6-di-n-butyl-DBT, carbazole, dibenzofuran, fluorene, biphenyl, DBT sulfone, benzothiophene, and naphthothiophene Bacillus subtilis ?
-
 ?
1.14.14.21 additional information coupled reaction with flavin reductase (TdsD) from thermophilic Paenibacillus sp. strain A11-2. Substrate specificity, overview. No activity with 4,6-di-n-propyl-DBT, 4,6-di-n-butyl-DBT, carbazole, dibenzofuran, fluorene, biphenyl, DBT sulfone, benzothiophene, and naphthothiophene Bacillus subtilis WU-S2B ?
-
 ?
1.14.14.21 thioxanthen-9-one + 2 FMNH2 + 2 O2
-
 Bacillus subtilis ?
-
 ?
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Bacillus subtilis 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
1.14.14.22 additional information coupled reaction with flavin reductase (TdsD) from thermophilic Paenibacillus sp. strain A11-2 Bacillus subtilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.21 homotetramer 4 * 45000, SDS-PAGE Bacillus subtilis
1.14.14.22 homotetramer 4 * 45000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.14.14.21 BdsC
-
 Bacillus subtilis
1.14.14.21 DBT monooxygenase
-
 Bacillus subtilis
1.14.14.22 BdsA
-
 Bacillus subtilis
1.14.14.22 DBT sulfone monooxygenase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.21 50
-
-
 Bacillus subtilis
1.14.14.22 50
-
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.21 50
-
 purified enzyme, 30 min, 80% activity remaning Bacillus subtilis
1.14.14.22 50
-
 purified enzyme, 30 min, 30% activity remaning Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.21 8
-
-
 Bacillus subtilis
1.14.14.22 7.5
-
-
 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.14.21 8 11 purified enzyme, stable at Bacillus subtilis
1.14.14.22 6 10 purified enzyme, stable at Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.21 FMNH2
-
 Bacillus subtilis
1.14.14.22 FMNH2
-
 Bacillus subtilis