EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.5.3 | copper | Mo/Cu-containing enzyme active site | Afipia carboxidovorans | |
1.2.5.3 | Fe-S cluster | proximal Fe-S cluster I and distal Fe-S cluster II | Afipia carboxidovorans | |
1.2.5.3 | Molybdenum | Mo/Cu-containing enzyme active site. The overall configuration of the binuclear center is L-MoVIO2-microS-CuI-Cys388, with L representing a bidentate pyranopterin cofactor common to all molybdenum enzymes other than nitrogenase | Afipia carboxidovorans | |
1.2.5.3 | additional information | the binuclear active site contains copper as well as molybdenum | Afipia carboxidovorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.5.3 | CO + ubiquinone + H2O | Afipia carboxidovorans | - |
CO2 + ubiquinol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.5.3 | Afipia carboxidovorans | P19919 and P19920 and P19921 | genes coxL, coxM, and coxS; genes coxS, coxM, and coxL | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.5.3 | CO + ubiquinone + H2O | - |
Afipia carboxidovorans | CO2 + ubiquinol | - |
? | |
1.2.5.3 | CO + ubiquinone + H2O | oxidation of carbon monoxide occurs at the binuclear center with reducing equivalents passed from the redox-active molybdenum to the proximal Fe-S cluster I to the distal Fe-S cluster II and finally to the FAD cofactor | Afipia carboxidovorans | CO2 + ubiquinol | - |
? | |
1.2.5.3 | additional information | analysis of mechanism of H2 oxidation, which involves initial binding of H2 to the copper of the binuclear center, displacing the bound water, followed by sequential deprotonation through a copper-hydride intermediate to reduce the binuclear center.The enzyme can be reduced by H2 with a limiting rate constant of 5.3/s and a dissociation constant Kd of 0.525 mM, steady-state and stopped-flow rapid reaction kinetics, overview | Afipia carboxidovorans | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.5.3 | heterohexamer | (alphabetagamma)2 hexamer, with a large subunit (coxL, 88.7 kDa) containing the binuclear active site, a medium subunit (coxM, 30.2 kDa) with FAD, and a small subunit (coxS, 30.2 kDa) containing two spinach ferredoxin-like [2Fe-2S] clusters | Afipia carboxidovorans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.5.3 | CO dehydrogenase | - |
Afipia carboxidovorans |
1.2.5.3 | molybdenum/copper-containing carbon monoxide dehydrogenase | - |
Afipia carboxidovorans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.5.3 | 25 | - |
assay at | Afipia carboxidovorans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.5.3 | 7.2 | - |
assay at | Afipia carboxidovorans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.5.3 | FAD | - |
Afipia carboxidovorans | |
1.2.5.3 | Mo/Cu cofactor | - |
Afipia carboxidovorans | |
1.2.5.3 | ubiquinone | - |
Afipia carboxidovorans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.2.5.3 | evolution | the Mo- and Cu-containing CO dehydrogenase from Oligotropha carboxydovorans is both mechanistically and structurally distinct from the extremely O2-sensitive Ni- and Fe-containing CO dehydrogenase from organisms such as Moorella thermoacetica or Methanosarcina barkerii. On the basis of overall architecture and sequence homology, the Mo/Cu CO dehydrogenase belongs to the xanthine oxidase family of enzymes but is unique among members of this large and broadly distributed family in several regards: the reaction catalyzed is not formally a hydroxylation reaction involving hydride abstraction from substrate. The enzyme utilizes ubiquinone as the oxidizing substrate rather than O2 or NADas oxidizing substrate, and, most significantly, its unique binuclear active site contains copper as well as molybdenum | Afipia carboxidovorans |