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Literature summary extracted from

  • Liu, L.; Wu, J.F.; Ma, Y.F.; Wang, S.Y.; Zhao, G.P.; Liu, S.J.
    A novel deaminase involved in chloronitrobenzene and nitrobenzene degradation with Comamonas sp. strain CNB-1 (2007), J. Bacteriol., 189, 2677-2682.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.99.11
-
 Comamonas testosteroni

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.99.11 Ca2+ 2 mM Comamonas testosteroni
3.5.99.11 Cu2+ 2 mM, complete inhibition Comamonas testosteroni
3.5.99.11 diethyl dicarbonate 10 mM Comamonas testosteroni
3.5.99.11 Mg2+ 2 mM Comamonas testosteroni
3.5.99.11 Ni2+ 2 mM, complete inhibition Comamonas testosteroni
3.5.99.11 p-chloromercuribenzoate 0.2 mM, complete inhibition Comamonas testosteroni
3.5.99.11 Zn2+ 2 mM, complete inhibition Comamonas testosteroni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.99.11 0.0039
-
 2-aminomuconate pH and temperature not specified in the publication Comamonas testosteroni
3.5.99.11 0.0088
-
 2-amino-5-chloromuconate pH and temperature not specified in the publication Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.99.11 28000
-
 1 * 28000, SDS-PAGE Comamonas testosteroni
3.5.99.11 30000
-
 gel filtration Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.99.11 2-aminomuconate + H2O Comamonas testosteroni the enzyme is involved in chloronitrobenzene and nitrobenzene degradation (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3
-
 ?
3.5.99.11 2-aminomuconate + H2O Comamonas testosteroni CNB-1 the enzyme is involved in chloronitrobenzene and nitrobenzene degradation (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.11 Comamonas testosteroni
-
-
-
3.5.99.11 Comamonas testosteroni CNB-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.99.11
-
 Comamonas testosteroni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.99.11 0.147
-
 substrate: 2-aminomuconate, pH and temperature not specified in the publication Comamonas testosteroni
3.5.99.11 0.196
-
 substrate: 2-amino-5-chloromuconate, pH and temperature not specified in the publication Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.11 2-amino-5-chloromuconate + H2O
-
 Comamonas testosteroni 2-hydroxy-5-chloromuconate + NH3
-
 ?
3.5.99.11 2-amino-5-chloromuconate + H2O
-
 Comamonas testosteroni CNB-1 2-hydroxy-5-chloromuconate + NH3
-
 ?
3.5.99.11 2-aminomuconate + H2O the enzyme is involved in chloronitrobenzene and nitrobenzene degradation Comamonas testosteroni (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3
-
 ?
3.5.99.11 2-aminomuconate + H2O 2-aminomuconate i.e. (2Z,4E)-2-aminohexa-2,4-dienedioate Comamonas testosteroni (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate i.e. (2Z,4E)-2-hydroxymuconate ?
3.5.99.11 2-aminomuconate + H2O the enzyme is involved in chloronitrobenzene and nitrobenzene degradation Comamonas testosteroni CNB-1 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3
-
 ?
3.5.99.11 2-aminomuconate + H2O 2-aminomuconate i.e. (2Z,4E)-2-aminohexa-2,4-dienedioate Comamonas testosteroni CNB-1 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate i.e. (2Z,4E)-2-hydroxymuconate ?

Subunits

EC Number Subunits Comment Organism
3.5.99.11 monomer 1 * 28000, SDS-PAGE Comamonas testosteroni

Synonyms

EC Number Synonyms Comment Organism
3.5.99.11 cnbZ
-
 Comamonas testosteroni

General Information

EC Number General Information Comment Organism
3.5.99.11 metabolism the enzyme is involved in chloronitrobenzene and nitrobenzene degradation Comamonas testosteroni