Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Porat, I.; Vinogradov, M.; Vyazmensky, M.; Lu, C.D.; Chipman, D.M.; Abdelal, A.T.; Barak, Z.
    Cloning and characterization of acetohydroxyacid synthase from Bacillus stearothermophilus (2004), J. Bacteriol., 186, 570-574.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6
-
 Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 sulfometuron methyl
-
 Geobacillus stearothermophilus
2.2.1.6 valine
-
 Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 8.8
-
 pyruvate holoenzyme, pH 8.0, 60°C Geobacillus stearothermophilus
2.2.1.6 12
-
 pyruvate large subunit, pH 8.0, 60°C Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ K0.5 value for holoenzyme 0.02 mM, for the isolated large subunit 1.3 mM Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 18700
-
 x * 63300, large subunit IlvB, plus x * 18700, small subunit IlvN, SDS-PAGE and calculated. Geobacillus stearothermophilus
2.2.1.6 63300
-
 x * 63300, large subunit IlvB, plus x * 18700, small subunit IlvN, SDS-PAGE and calculated. Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Geobacillus stearothermophilus Q8RL88 and Q8RL87 Q8RL88 i.e. large subunit IlvB, Q8RL87 i.e. small subunit IlvN
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.2.1.6 the isolated large subunit has about one-third the activity of the holoenzyme, but it can be reconstituted to nearly complete activity by the addition of purified small subunits Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 3.12
-
 large subunit, pH 7.9, 55°C Geobacillus stearothermophilus
2.2.1.6 9.16
-
 holoenzyme, pH 7.9, 55°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Geobacillus stearothermophilus (S)-acetolactate + CO2
-
 ?
2.2.1.6 2-oxobutanoate + pyruvate
-
 Geobacillus stearothermophilus 2-hydroxy-2-methyl-3-oxopentanoate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 multimer x * 63300, large subunit IlvB, plus x * 18700, small subunit IlvN, SDS-PAGE and calculated. Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.6 55
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.6 65
-
 hlaf-life 5 min Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 7.6
-
 holoenzyme Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD
-
 Geobacillus stearothermophilus
2.2.1.6 thiamine diphosphate K0.5 value for holoenzyme 0.0055 mM, for the isolated large subunit 0.24 mM Geobacillus stearothermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.2.1.6 0.012
-
 sulfometuron methyl holoenzyme, pH 7.9, 55°C Geobacillus stearothermophilus
2.2.1.6 0.018
-
 valine holoenzyme, pH 8.0, 60°C Geobacillus stearothermophilus