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Literature summary extracted from

  • Qiu, J.; Wang, D.; Ma, Y.; Jiang, T.; Xin, Y.
    Identification and characterization of serine acetyltransferase encoded by the Mycobacterium tuberculosis Rv2335 gene (2013), Int. J. Mol. Med., 31, 1229-1233.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.30 gene cysE or Rv2335, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.30 additional information
-
 additional information Michaelis-Menten kinetics, overview Mycobacterium tuberculosis
2.3.1.30 0.0264
-
 L-serine pH 7.5, 37°C, recombinant His-tagged enzyme Mycobacterium tuberculosis
2.3.1.30 0.0513
-
 acetyl-CoA pH 7.5, 37°C, recombinant His-tagged enzyme Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.30 additional information Mg2+ has no effect on the enzyme activity Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.30 24600
-
 x * 30000, recombinant His-tagged enzyme, SDS-PAGE, x * 24600, about, sequence calculation Mycobacterium tuberculosis
2.3.1.30 30000
-
 x * 30000, recombinant His-tagged enzyme, SDS-PAGE, x * 24600, about, sequence calculation Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Mycobacterium tuberculosis
-
 CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine Mycobacterium tuberculosis H37Rv
-
 CoA + O-acetyl-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Mycobacterium tuberculosis P95231 gene Rv2335 or cysE
-
2.3.1.30 Mycobacterium tuberculosis H37Rv P95231 gene Rv2335 or cysE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.30 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.30 10.66
-
 purified recombinant His-tagged enzyme, pH 7.5, 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Mycobacterium tuberculosis CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine
-
 Mycobacterium tuberculosis H37Rv CoA + O-acetyl-L-serine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.30 ? x * 30000, recombinant His-tagged enzyme, SDS-PAGE, x * 24600, about, sequence calculation Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.30 CysE
-
 Mycobacterium tuberculosis
2.3.1.30 serine acetyltransferase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.30 37
-
 recombinant enzyme Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.30 20 80 50% of maximal activity at 20°C and 80°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.30 7.5
-
 recombinant enzyme Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.30 5 10 50% of maximal activity at pH 5.0 and pH 10.0 Mycobacterium tuberculosis