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Literature summary extracted from

  • Fan, S.; Li, D.F.; Wang, D.C.; Fleming, J.; Zhang, H.; Zhou, Y.; Zhou, L.; Zhou, J.; Chen, T.; Chen, G.; Zhang, X.E.; Bi, L.
    Structure and function of Mycobacterium smegmatis 7-keto-8-aminopelargonic acid (KAPA) synthase (2015), Int. J. Biochem. Cell Biol., 58, 71-80.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.47 drug development the enzyme is a target for development of drugs against mycobac-terial pathogens Mycolicibacterium smegmatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.47 gene bioF, the enzyme coding gene is located between genes bioA(MSMEG 3188) and bioD (MSMEG 3190), recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3) Mycolicibacterium smegmatis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.47 the crystal structure of MsBioF is solved by molecular replacement using the structure of EcAONS, PDB code 1BS0, as the search model Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.47 pimeloyl-[acyl-carrier protein] + L-alanine Mycolicibacterium smegmatis
-
 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
 ?
2.3.1.47 pimeloyl-[acyl-carrier protein] + L-alanine Mycolicibacterium smegmatis ATCC 700084
-
 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.47 Mycolicibacterium smegmatis A0QX65 gene bioF
-
2.3.1.47 Mycolicibacterium smegmatis ATCC 700084 A0QX65 gene bioF
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.47 pimeloyl-[acyl-carrier protein] + L-alanine
-
 Mycolicibacterium smegmatis 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
 ?
2.3.1.47 pimeloyl-[acyl-carrier protein] + L-alanine
-
 Mycolicibacterium smegmatis ATCC 700084 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.47 dimer the structure reveals an incomplete dimer, three-dimensional structure of apoenzyme, overview Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.47 7-keto-8-aminopelargonic acid synthase
-
 Mycolicibacterium smegmatis
2.3.1.47 BioF
-
 Mycolicibacterium smegmatis
2.3.1.47 KAPA synthase
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.47 pyridoxal 5'-phosphate dependent on, binding site residues His129, Lys235, and His200 are essential for MsBioF activity in vivo Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
2.3.1.47 metabolism the enzyme is essential for mycobacterial growth and plays a key role as the gatekeeper of the biotin biosynthesis pathway, catalyzing the first committed step, decarboxylative condensation of L-alanine with pimeloyl-CoA to form 7-oxo-8-aminopelargonic acid Mycolicibacterium smegmatis
2.3.1.47 additional information the structure reveals an incomplete dimer, and the active site organization is similar to, but distinct from Escherichia coli 8-amino-7-oxononanoate synthase (EcAONS). Pyridoxal 5'-phosphate binding site residues His129, Lys235, and His200 are essential for MsBioF activity in vivo and residue Glu171 plays an important, but not essential role in MsBioF activity. The N-terminus (residues 1-37) is also essential for MsBioF activity in vivo, structure homology modeling and structure-function analysis of MsBioF, overview Mycolicibacterium smegmatis