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Literature summary extracted from

  • Taus, C.; Lucini, C.; Sato, T.; Furukawa, K.; Grabherr, R.; Staudacher, E.
    Expression and characterization of the first snail-derived UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (2013), Glycoconj. J., 30, 825-833.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.41 expressed in Sf9 insect cells Biomphalaria glabrata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.41 acetonitrile 10% (v/v) acetonitrile reduces enzyme activity to about 60% Biomphalaria glabrata
2.4.1.41 Cu2+
-
 Biomphalaria glabrata
2.4.1.41 EDTA no activity in the presence of EDTA Biomphalaria glabrata
2.4.1.41 Mn2+ inhibitory at concentrations higher than 80 mM Biomphalaria glabrata
2.4.1.41 additional information addition of 5% (v/v) of acetonitrile or the addition of up to 10% (v/v) methanol or glycerol has no negative effect on enzyme activity Biomphalaria glabrata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.41 0.046
-
 UDP-N-acetyl-alpha-D-galactosamine with polypeptide Muc2 as cosubstrate, at pH 6.0 and 37°C Biomphalaria glabrata
2.4.1.41 0.064
-
 polypeptide Muc2 at pH 6.0 and 37°C Biomphalaria glabrata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.41 Ca2+ stimulates activity to a lesser extent than Mn2+ Biomphalaria glabrata
2.4.1.41 Co2+ stimulates activity to a lesser extent than Mn2+ Biomphalaria glabrata
2.4.1.41 Mg2+ stimulates activity to a lesser extent than Mn2+ Biomphalaria glabrata
2.4.1.41 Mn2+ best stimulator of activity Biomphalaria glabrata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.41 65000
-
 x * 65000, SDS-PAGE Biomphalaria glabrata

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Biomphalaria glabrata
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.41 glycoprotein the enzyme is O-glycosylated but does not have any putative N-glycosylation sites Biomphalaria glabrata

Storage Stability

EC Number Storage Stability Organism
2.4.1.41 -20 to 22°C, 72 h, no loss of activity Biomphalaria glabrata
2.4.1.41 37°C, 72 h, more than 90% loss of activity Biomphalaria glabrata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 2 UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1a sequence APPAHGVTSAPDTRPAPGC Biomphalaria glabrata 2 UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1a
-
 ?
2.4.1.41 2 UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1a' sequence AHGVTSAPDTR Biomphalaria glabrata 2 UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1a'
-
 ?
2.4.1.41 UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc2 best acceptor polypeptide substrate with sequence PTTTPITTTTTVTPTPTPTGTQTK Biomphalaria glabrata UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc2
-
 ?
2.4.1.41 UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc5Ac second best acceptor polypeptide with sequence GTTPSPVPTTSTTSAP Biomphalaria glabrata UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc5Ac
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.41 ? x * 65000, SDS-PAGE Biomphalaria glabrata

Synonyms

EC Number Synonyms Comment Organism
2.4.1.41 ppGalNAcT
-
 Biomphalaria glabrata
2.4.1.41 UDP-GalNAc:polypeptide GalNAc transferase
-
 Biomphalaria glabrata
2.4.1.41 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase
-
 Biomphalaria glabrata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.41 37
-
 optimal incubation temperature for short assays up to 2 h Biomphalaria glabrata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.41 6 6.5
-
 Biomphalaria glabrata

General Information

EC Number General Information Comment Organism
2.4.1.41 metabolism the enzyme catalyzes the first step in mucin-type O-glycosylation Biomphalaria glabrata