Literature summary extracted from

Zhang, Y.; Dai, Z.; Krivoruchko, A.; Chen, Y.; Siewers, V.; Nielsen, J.
Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth (2015), FEMS Yeast Res., 15, fov024.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.3.1.54	reduced ferredoxin	reduced flavodoxin is te better cofactor	Escherichia coli
2.3.1.54	reduced flavodoxin	a better electron donor than reduced ferredoxin	Escherichia coli

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.3.1.54	gene pflB, the enzyme and its activating enzyme from Escherichia coli are expressed in a Saccharomyces cerevisiae strain IMI076 lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1. Enzyme PFL is expressed with two different electron donors, reduced ferredoxin or reduced flavodoxin, respectively. The coexpression of either of these electron donors has a positive effect on growth under aerobic conditions, indicating increased activity of PFL.	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.1.54	Escherichia coli	P09373	gene pflB	-

Synonyms

EC Number	Synonyms	Comment	Organism
2.3.1.54	PFL	-	Escherichia coli
2.3.1.54	pyruvate formate lyase	-	Escherichia coli

Expression

EC Number	Organism	Comment	Expression
2.3.1.54	Escherichia coli	coexpression of either of the electron donors reduced ferredoxin or reduced flavodoxin has a positive effect on growth under aerobic conditions, indicating increased activity of PFL. with a higher final biomass concentration and a significant increase in transcription of formate dehydrogenases	up

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Release 2023.1 (January 2023)