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Literature summary extracted from

  • Akita, H.; Watanabe, M.; Suzuki, T.; Nakashima, N.; Hoshino, T.
    Molecular cloning and characterization of two YGL039w genes encoding broad specificity NADPH-dependent aldehyde reductases from Kluyveromyces marxianus strain DMB1 (2015), FEMS Microbiol. Lett., 362, fnv116.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.283 expression in Escherichia coli Kluyveromyces marxianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.283 0.028
-
 NADPH pH 6.0, 25° Kluyveromyces marxianus
1.1.1.283 0.034
-
 NADPH pH 6.0, 25° Kluyveromyces marxianus
1.1.1.283 0.43
-
 methyl glyoxal pH 6.0, 25° Kluyveromyces marxianus
1.1.1.283 1.6
-
 methyl glyoxal pH 6.0, 25° Kluyveromyces marxianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.283 35800
-
 gel filtration Kluyveromyces marxianus
1.1.1.283 36900
-
 gel filtration Kluyveromyces marxianus
1.1.1.283 37000
-
 1 * 37000, SDS-PAGE Kluyveromyces marxianus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.283 Kluyveromyces marxianus A0A0E4AX59
-
-
1.1.1.283 Kluyveromyces marxianus A0A0E4AY21
-
-
1.1.1.283 Kluyveromyces marxianus DMB1 A0A0E4AX59
-
-
1.1.1.283 Kluyveromyces marxianus DMB1 A0A0E4AY21
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.283 0.96
-
 substrate methylglyoxal, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.283 1.1
-
 substrate p-anisaldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.283 2.1
-
 substrate octanal, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.283 2.1
-
 substrate valeraldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.283 3
-
 substrate heptanal, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.283 3.2
-
 substrate methyl glyoxal, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.283 3.3
-
 substrate isovaleraldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.283 4.2
-
 substrate isovaleraldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.283 4.5
-
 substrate p-anisaldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.283 5.3
-
 substrate octanal, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.283 5.9
-
 substrate valeraldehyde, pH 6.0, 25°C Kluyveromyces marxianus
1.1.1.283 6.9
-
 substrate heptanal, pH 6.0, 25°C Kluyveromyces marxianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.283 heptanal + NADPH + H+
-
 Kluyveromyces marxianus heptan-1-ol + NADP+
-
 ir
1.1.1.283 heptanal + NADPH + H+
-
 Kluyveromyces marxianus DMB1 heptan-1-ol + NADP+
-
 ir
1.1.1.283 isovaleraldehyde + NADPH + H+
-
 Kluyveromyces marxianus isoamyl alcohol + NADP+
-
 ir
1.1.1.283 isovaleraldehyde + NADPH + H+
-
 Kluyveromyces marxianus DMB1 isoamyl alcohol + NADP+
-
 ir
1.1.1.283 methyl glyoxal + NADPH + H+
-
 Kluyveromyces marxianus ? + NADP+
-
 ir
1.1.1.283 methyl glyoxal + NADPH + H+
-
 Kluyveromyces marxianus DMB1 ? + NADP+
-
 ir
1.1.1.283 additional information enzyme shows strong activities toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine, D-lactate, L-lactate or pyruvate. Enzyme has no NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol Kluyveromyces marxianus ?
-
 ?
1.1.1.283 additional information enzyme shows strong activities toward linear aldehydes, such as heptanal, valeraldehyde and octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine, D-lactate, L-lactate or pyruvate. Enzyme has no NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol Kluyveromyces marxianus ?
-
 ?
1.1.1.283 additional information enzyme shows strong activities toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine, D-lactate, L-lactate or pyruvate. Enzyme has no NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol Kluyveromyces marxianus DMB1 ?
-
 ?
1.1.1.283 additional information enzyme shows strong activities toward linear aldehydes, such as heptanal, valeraldehyde and octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine, D-lactate, L-lactate or pyruvate. Enzyme has no NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol Kluyveromyces marxianus DMB1 ?
-
 ?
1.1.1.283 octanal + NADPH + H+
-
 Kluyveromyces marxianus octan-1-ol + NADP+
-
 ir
1.1.1.283 octanal + NADPH + H+
-
 Kluyveromyces marxianus DMB1 octan-1-ol + NADP+
-
 ir
1.1.1.283 p-anisaldehyde + NADPH + H+
-
 Kluyveromyces marxianus p-anisalcohol + NADP+
-
 ir
1.1.1.283 pentanal + NADPH + H+
-
 Kluyveromyces marxianus pentan-1-ol + NADP+
-
 ir
1.1.1.283 valeraldehyde + NADPH + H+
-
 Kluyveromyces marxianus amyl alcohol + NADP+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.283 monomer 1 * 37000, SDS-PAGE Kluyveromyces marxianus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.283 YGL039w1
-
 Kluyveromyces marxianus
1.1.1.283 YGL039w2
-
 Kluyveromyces marxianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.283 45
-
-
 Kluyveromyces marxianus
1.1.1.283 50
-
-
 Kluyveromyces marxianus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.283 40
-
 below 40°C, 30 min, 80% residual activity Kluyveromyces marxianus
1.1.1.283 50
-
 30 min, complete loss of activity Kluyveromyces marxianus
1.1.1.283 50
-
 30 min, 26% residual activity Kluyveromyces marxianus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.283 2.5
-
 methyl glyoxal pH 6.0, 25° Kluyveromyces marxianus
1.1.1.283 2.67
-
 NADPH pH 6.0, 25° Kluyveromyces marxianus
1.1.1.283 3.67
-
 NADPH pH 6.0, 25° Kluyveromyces marxianus
1.1.1.283 4
-
 methyl glyoxal pH 6.0, 25° Kluyveromyces marxianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.283 6
-
-
 Kluyveromyces marxianus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.283 additional information no activity with NADH Kluyveromyces marxianus
1.1.1.283 additional information NADH is inert Kluyveromyces marxianus
1.1.1.283 NADPH
-
 Kluyveromyces marxianus