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Literature summary extracted from

  • Nihira, T.; Saito, Y.; Chiku, K.; Kitaoka, M.; Ohtsubo, K.; Nakai, H.
    Potassium ion-dependent trehalose phosphorylase from halophilic Bacillus selenitireducens MLS10 (2013), FEBS Lett., 587, 3382-3386.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.64 expressed in Escherichia coli BL21(DE3) cells Salisediminibacterium selenitireducens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.64 alpha,alpha-trehalose
-
 Salisediminibacterium selenitireducens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.64 0.14
-
 phosphate recombinant enzyme, at pH 6.5 and 30°C Salisediminibacterium selenitireducens
2.4.1.64 0.86
-
 alpha,alpha-trehalose recombinant enzyme, at pH 6.5 and 30°C Salisediminibacterium selenitireducens
2.4.1.64 2
-
 beta-D-glucose 1-phosphate recombinant enzyme, at pH 6.5 and 30°C Salisediminibacterium selenitireducens
2.4.1.64 5.5
-
 D-glucose recombinant enzyme, at pH 6.5 and 30°C Salisediminibacterium selenitireducens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.64 K+ essential for enzymatic activity. Decreasing potassium ion concentrations significantly reduce thermal and pH stabilities of the enzyme Salisediminibacterium selenitireducens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.64 87889
-
 2 * 87889, calculated from amino acid sequence Salisediminibacterium selenitireducens
2.4.1.64 88000
-
 2 * 88000, SDS-PAGE Salisediminibacterium selenitireducens
2.4.1.64 170000
-
 gel filtration Salisediminibacterium selenitireducens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.64 alpha,alpha-trehalose + phosphate Salisediminibacterium selenitireducens
-
 D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.64 alpha,alpha-trehalose + phosphate Salisediminibacterium selenitireducens MLS10
-
 D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.64 D-glucose + beta-D-glucose 1-phosphate Salisediminibacterium selenitireducens
-
 alpha,alpha-trehalose + phosphate
-
 r
2.4.1.64 D-glucose + beta-D-glucose 1-phosphate Salisediminibacterium selenitireducens MLS10
-
 alpha,alpha-trehalose + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.64 Salisediminibacterium selenitireducens D6XSD4
-
-
2.4.1.64 Salisediminibacterium selenitireducens MLS10 D6XSD4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.64 HisTrap column chromatography Salisediminibacterium selenitireducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.64 alpha,alpha-trehalose + phosphate
-
 Salisediminibacterium selenitireducens D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.64 alpha,alpha-trehalose + phosphate
-
 Salisediminibacterium selenitireducens MLS10 D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.64 D-glucose + beta-D-glucose 1-phosphate
-
 Salisediminibacterium selenitireducens alpha,alpha-trehalose + phosphate
-
 r
2.4.1.64 D-glucose + beta-D-glucose 1-phosphate
-
 Salisediminibacterium selenitireducens MLS10 alpha,alpha-trehalose + phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.64 homodimer 2 * 88000, SDS-PAGE Salisediminibacterium selenitireducens
2.4.1.64 homodimer 2 * 87889, calculated from amino acid sequence Salisediminibacterium selenitireducens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.64 Bsel_1207 recombinant protein Salisediminibacterium selenitireducens
2.4.1.64 trehalose phosphorylase
-
 Salisediminibacterium selenitireducens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.64 60
-
 D-glucose recombinant enzyme, at pH 6.5 and 30°C Salisediminibacterium selenitireducens
2.4.1.64 68
-
 beta-D-glucose 1-phosphate recombinant enzyme, at pH 6.5 and 30°C Salisediminibacterium selenitireducens
2.4.1.64 210
-
 alpha,alpha-trehalose recombinant enzyme, at pH 6.5 and 30°C Salisediminibacterium selenitireducens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.64 95
-
 alpha,alpha-trehalose recombinant enzyme, at pH 6.5 and 30°C Salisediminibacterium selenitireducens

General Information

EC Number General Information Comment Organism
2.4.1.64 metabolism the enzyme is involved in osmotic control through the biosynthesis of trehalose Salisediminibacterium selenitireducens