Literature summary extracted from
Zhao, W.; Zhou, X.; Lu, Y.; Peng, Y.; Lin, Z.; Lin, J.; Yang, L.; Yin, X.; Zhao, D.
Mycobacterium bovis ornithine carbamoyltransferase, MB1684, induces proinflammatory cytokine gene expression by activating NF-kappaB in macrophages (2014), DNA Cell Biol., 33, 311-319.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.1.3.3 |
gene MB1684, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS |
Mycobacterium tuberculosis variant bovis |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.1.3.3 |
39000 |
- |
x * 39000, untagged enzyme, SDS-PAGE, x * 41000, recombinant His-tagged enzyme, SDS-PAGE |
Mycobacterium tuberculosis variant bovis |
2.1.3.3 |
41000 |
- |
x * 39000, untagged enzyme, SDS-PAGE, x * 41000, recombinant His-tagged enzyme, SDS-PAGE |
Mycobacterium tuberculosis variant bovis |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.1.3.3 |
carbamoyl phosphate + L-ornithine |
Mycobacterium tuberculosis variant bovis |
- |
phosphate + L-citrulline |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.1.3.3 |
Mycobacterium tuberculosis variant bovis |
P0A5M9 |
gene MB1684 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.1.3.3 |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography |
Mycobacterium tuberculosis variant bovis |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.1.3.3 |
carbamoyl phosphate + L-ornithine |
- |
Mycobacterium tuberculosis variant bovis |
phosphate + L-citrulline |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.1.3.3 |
? |
x * 39000, untagged enzyme, SDS-PAGE, x * 41000, recombinant His-tagged enzyme, SDS-PAGE |
Mycobacterium tuberculosis variant bovis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.1.3.3 |
carbamoyltransferase |
- |
Mycobacterium tuberculosis variant bovis |
2.1.3.3 |
MB1684 |
- |
Mycobacterium tuberculosis variant bovis |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
2.1.3.3 |
physiological function |
the enzyme induces proinflammatory cytokine gene expression by activating NF-kappaB in macrophages of the bovine host. The enzyme plays a role in induction of upregulation of interferon-gamma and proinflammatory cytokines (interleukin-1beta, IL-6, and tumor necrosis factor-alpha) in Bos taurus Ana-1 macrophages |
Mycobacterium tuberculosis variant bovis |