EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.1.240 | biotechnology | large-scale production of plant metabolites via microbial approaches is a promising alternative to chemical synthesis and extraction from plant sources Development of an Escherichia coli system containing an artificial biosynthetic pathway, involving the enzyme, that produces methylated resveratrol analogues, such as pinostilbene (3,4'-dihydroxy-5-methoxystilbene), 3,5-dihydroxy-4'-methoxystilbene, 3,4'-dimethoxy-5-hydroxystilbene, and 3,5,4'-trimethoxystilbene, from simple carbon sources | Sorghum bicolor |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.240 | gene sbOMT3, recombinant expression of codon-optimized resveratrol O-methyltransferase genes sbOMT1 and sbOMT3 in Escherichia coli strain C41 (DE3), development of an artificial biosynthetic pathway that produces methylated resveratrol analogues, such as pinostilbene (3,4'-dihydroxy-5-methoxystilbene), 3,5-dihydroxy-4'-methoxystilbene, 3,4'-dimethoxy-5-hydroxystilbene, and 3,5,4'-trimethoxystilbene, from simple carbon sources in Escherichia coli | Sorghum bicolor |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.240 | additional information | development of an Escherichia coli system containing an artificial biosynthetic pathway that produces methylated resveratrol analogues, such as pinostilbene (3,4'-dihydroxy-5-methoxystilbene), 3,5-dihydroxy-4'-methoxystilbene, 3,4'-dimethoxy-5-hydroxystilbene, and 3,5,4'-trimethoxystilbene, from simple carbon sources. The artificial biosynthetic pathways contain a series of codon-optimized O-methyltransferase genes from sorghum in addition to the resveratrol biosynthetic genes | Sorghum bicolor |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.240 | S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene | Sorghum bicolor | i.e. pinostilbene | S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
2.1.1.240 | S-adenosyl-L-methionine + trans-resveratrol | Sorghum bicolor | - |
S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.240 | Sorghum bicolor | A8QW53 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.240 | S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene | i.e. pinostilbene | Sorghum bicolor | S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
2.1.1.240 | S-adenosyl-L-methionine + trans-resveratrol | - |
Sorghum bicolor | S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.240 | dimer | - |
Sorghum bicolor |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.240 | 5-pentadecatrienyl resorcinol O-methyltransferase | UniProt | Sorghum bicolor |
2.1.1.240 | resveratrol O-methyltransferase | - |
Sorghum bicolor |
2.1.1.240 | SbOMT3 | - |
Sorghum bicolor |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.240 | S-adenosyl-L-methionine | - |
Sorghum bicolor |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.240 | metabolism | two resveratrol O-methyltransferase genes (sbOMT1 and sbOMT3) from Sorghum bicolor are capable of using resveratrol as a substrate that yields methylated analogues of resveratrol. The sbOMT3 O-methyltransferase catalyzes the A-ring specific 3,5-bis-O-methylation of resveratrol, which in turn yields pterostilbene (3,5-dimethoxy-4'-hydroxystilbene) in coexpression with a stilbene synthase from Arachis hypogaea. In addition, resveratrol O-methyltransferase sbOMT1, which has a potential as eugenol O-methyltransferase, predominantly catalyzes the resveratrol B-ring (4'-O-methylation), which yields 3,5-dihydroxy-4'-methoxystilbene | Sorghum bicolor |