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Literature summary extracted from

  • Frommhagen, M; Sforza, S.; Westphal, A.H.; Visser, J.; Hinz, S.W.; Koetsier, M.J.; van Berkel, W.J.; Gruppen, H.; Kabel, M.A.
    Discovery of the combined oxidative cleavage of plant xylan and cellulose by a new fungal polysaccharide monooxygenase (2015), Biotechnol. Biofuels, 17, 101.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.54 expressed and produced in a protease/(hemi-) cellulase-free Myceliophthora thermophila C1 strain Thermothelomyces thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.54 22770
-
 mass spectrometry Thermothelomyces thermophilus
1.14.99.54 23000
-
 SDS-PAGE Thermothelomyces thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.54 additional information Thermothelomyces thermophilus The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone ?
-
 ?
1.14.99.54 additional information Thermothelomyces thermophilus C1 The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone ?
-
 ?
1.14.99.54 [(1->4)-beta-D-glucosyl]n+m + AH2 + O2 Thermothelomyces thermophilus
-
 [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + A + H2O
-
 ?
1.14.99.54 [(1->4)-beta-D-glucosyl]n+m + AH2 + O2 Thermothelomyces thermophilus C1
-
 [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.54 Thermothelomyces thermophilus A0A0H4K9X4 Thielavia heterothallica
-
1.14.99.54 Thermothelomyces thermophilus C1 A0A0H4K9X4 Thielavia heterothallica
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.99.54 methylation the enzyme contains an N-methylated histidine at the N-terminus Thermothelomyces thermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.54
-
 Thermothelomyces thermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.99.54 [(1->4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + acceptor + H2O (1) the D-glucono-1,5-lactone at the reducing end of the cellulose chain quickly hydrolyzes spontaneuosly to the aldonic acid Thermothelomyces thermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.54 additional information The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone Thermothelomyces thermophilus ?
-
 ?
1.14.99.54 additional information The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone Thermothelomyces thermophilus C1 ?
-
 ?
1.14.99.54 [(1->4)-beta-D-glucosyl]n+m + AH2 + O2
-
 Thermothelomyces thermophilus [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + A + H2O
-
 ?
1.14.99.54 [(1->4)-beta-D-glucosyl]n+m + AH2 + O2
-
 Thermothelomyces thermophilus C1 [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + A + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.54 LPMO ambiguous Thermothelomyces thermophilus
1.14.99.54 LPMO9A
-
 Thermothelomyces thermophilus
1.14.99.54 lytic polysaccharide monooxygenase ambiguous Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.54 50
-
 assay temperature Thermothelomyces thermophilus