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Literature summary extracted from

  • Ohshiro, T.; Nakura, S.; Ishii, Y.; Kino, K.; Kirimura, K.; Izumi, Y.
    Novel reactivity of dibenzothiophene monooxygenase from Bacillus subtilis WU-S2B (2009), Biosci. Biotechnol. Biochem., 73, 2128-2130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.21 gene bdsC, recombinant expression in Escherichia coli strain BL21(DE3)/pET21-a/bdsC Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2 Bacillus subtilis
-
 dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2 Bacillus subtilis WU-S2B
-
 dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.21 Bacillus subtilis
-
 gene bdsC
-
1.14.14.21 Bacillus subtilis WU-S2B
-
 gene bdsC
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.21 2,7-diethylbenzothiophene + FMNH2 + O2 13% of the activity with dibenzothiophene Bacillus subtilis ?
-
 ?
1.14.14.21 2,7-diethylbenzothiophene + FMNH2 + O2 13% of the activity with dibenzothiophene Bacillus subtilis WU-S2B ?
-
 ?
1.14.14.21 2-ethylbenzothiophene + FMNH2 + O2 28% of the activity with dibenzothiophene Bacillus subtilis ?
-
 ?
1.14.14.21 2-ethylbenzothiophene + FMNH2 + O2 28% of the activity with dibenzothiophene Bacillus subtilis WU-S2B ?
-
 ?
1.14.14.21 2-methylbenzothiophene + FMNH2 + O2 209% of the activity with dibenzothiophene Bacillus subtilis ?
-
 ?
1.14.14.21 2-methylbenzothiophene + FMNH2 + O2 209% of the activity with dibenzothiophene Bacillus subtilis WU-S2B ?
-
 ?
1.14.14.21 3-methylbenzothiophene + FMNH2 + O2 less than 10% of the activity with dibenzothiophene Bacillus subtilis ?
-
 ?
1.14.14.21 5-methylbenzothiophene + FMNH2 + O2 33% of the activity with dibenzothiophene Bacillus subtilis ?
-
 ?
1.14.14.21 7-ethylbenzothiophene + FMNH2 + O2 less than 10% of the activity with dibenzothiophene Bacillus subtilis ?
-
 ?
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2
-
 Bacillus subtilis WU-S2B dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 additional information the enzyme utilizes aromatic compounds, not having sulfur atoms, as substrates. It acts on indole, converting it to indoxyl and isatin, and its derivatives to form indigoid pigments, and also utilizes indoline and phenoxazine. Indigo derivative formation by BdsC using methylindoles as substrates. Enzyme BdsC exhibits activity toward benzothiophene derivatives but not benzothiophene itself showing a wide reactivity toward aromatic compounds. To measure BdsC activity, flavin reductase (DszD) from another desulfurizing species, Rhodococcus erythropolis strain D-1, is used. Sulfone formations, product analysis by GC-MS Bacillus subtilis ?
-
 ?
1.14.14.21 additional information the enzyme utilizes aromatic compounds, not having sulfur atoms, as substrates. It acts on indole, converting it to indoxyl and isatin, and its derivatives to form indigoid pigments, and also utilizes indoline and phenoxazine. Indigo derivative formation by BdsC using methylindoles as substrates. Enzyme BdsC exhibits activity toward benzothiophene derivatives but not benzothiophene itself showing a wide reactivity toward aromatic compounds. To measure BdsC activity, flavin reductase (DszD) from another desulfurizing species, Rhodococcus erythropolis strain D-1, is used. Sulfone formations, product analysis by GC-MS Bacillus subtilis WU-S2B ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.21 BdsC
-
 Bacillus subtilis
1.14.14.21 dibenzothiophene monooxygenase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.21 30
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.21 7
-
 assay at Bacillus subtilis

General Information

EC Number General Information Comment Organism
1.14.14.21 metabolism the dibenzothiophene (DBT) desulfurization enzyme system consists of three enzymes: DBT monooxygenase, DBT sulfone monooxygenase, and 2'-hydroxybiphenyl 2-sulfinate desulfinase Bacillus subtilis