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Literature summary extracted from

  • Ehrhardt, M.; Gerber, A.; Zapp, J.; Hannemann, F.; Bernhardt, R.
    Human CYP27A1 catalyzes hydroxylation of beta-sitosterol and ergosterol (2016), Biol. Chem., 397, 513-518.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.15 gene CYP27A1, recombinant expression of His-tagged enzyme in Bacillus megaterium Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.15 mitochondrion
-
 Homo sapiens 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.15 Homo sapiens Q02318 gene CYP27A1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.15 recombinant His-tagged enzyme from Bacillus megaterium Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.15 enterocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.15 beta-sitosterol + reduced adrenodoxin + O2
-
 Homo sapiens 26-hydroxy-beta-sitosterol + 29-hydroxy-beta-sitosterol + oxidized adrenodoxin + H2O
-
 ?
1.14.15.15 cholesterol + reduced adrenodoxin + O2
-
 Homo sapiens 26-hydroxycholesterol + oxidized adrenodoxin + H2O
-
 ?
1.14.15.15 ergosterol + reduced adrenodoxin + O2
-
 Homo sapiens 24-hydroxyergosterol + 26-hydroxyergosterol + 28-hydroxyergosterol + oxidized adrenodoxin + H2O
-
 ?
1.14.15.15 additional information human CYP27A1 catalyzes hydroxylation of beta-sitosterol and ergosterol, CYP27A1 catalyzes the 26-hydroxylation of cholesterol to form the physiologically active product 26-hydroxycholesterol. CYP27A1 is also able to accept other steroids with structural similarity to cholesterol as substrates such as vitamin D3 and its precursor 7-dehydrocholesterol. NMR spectroscopic product analysis Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.15 CYP27A1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.15 30
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.15 adrenodoxin
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.15.15 physiological function the enzyme forms 26-hydroxycholesterolthat acts as an endogenous selective estrogen receptor modulator and has, among multiple other functions, a strong influence on the promotion of estrogen receptor dependent breast cancer, the bone mineralization as well as the cardiovascular system Homo sapiens