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Literature summary extracted from

  • Gangoiti, J.; van Leeuwen, S.S.; Vafiadi, C.; Dijkhuizen, L.
    The Gram-negative bacterium Azotobacter chroococcum NCIMB 8003 employs a new glycoside hydrolase family 70 4,6-alpha-glucanotransferase enzyme (GtfD) to synthesize a reuteran like polymer from maltodextrins and starch (2016), Biochim. Biophys. Acta, 1860, 1224-1236.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.B34 86400
-
x * 90000, SDS-PAGE, x * 86400, calculated Azotobacter chroococcum
2.4.1.B34 90000
-
x * 90000, SDS-PAGE, x * 86400, calculated Azotobacter chroococcum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B34 Azotobacter chroococcum A0A0C4WTK3
-
-
2.4.1.B34 Azotobacter chroococcum NCIMB 8003 A0A0C4WTK3
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.B34 6.6
-
pH 6.5, 50°C Azotobacter chroococcum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B34 amylose V
-
Azotobacter chroococcum ? GtfB produces a high molecular mass polymer with a molecular mass about 80 times greater than that of the starting amylose V ?
2.4.1.B34 amylose V + maltose
-
Azotobacter chroococcum maltotriose + maltotetraose + ?
-
?
2.4.1.B34 Maltoheptaose
-
Azotobacter chroococcum ?
-
?
2.4.1.B34 Maltoheptaose
-
Azotobacter chroococcum NCIMB 8003 ?
-
?
2.4.1.B34 Maltohexaose
-
Azotobacter chroococcum ?
-
?
2.4.1.B34 Maltohexaose
-
Azotobacter chroococcum NCIMB 8003 ?
-
?
2.4.1.B34 maltopentaose
-
Azotobacter chroococcum ?
-
?
2.4.1.B34 maltose
-
Azotobacter chroococcum ?
-
?
2.4.1.B34 Maltotetraose
-
Azotobacter chroococcum ?
-
?
2.4.1.B34 Maltotetraose
-
Azotobacter chroococcum NCIMB 8003 ?
-
?
2.4.1.B34 maltotriose
-
Azotobacter chroococcum ?
-
?
2.4.1.B34 additional information GtfD shows clear hydrolase/transglycosylase activity with malto-oligosaccharides with degree of polymerzation of 3 to 7 and forms a range of shorter and longer oligosaccharides. The enzyme is unable to synthesize consecutive alpha1->6 glucosidic bonds. Instead, it forms a high molecular mass and branched alpha-glucan with alternating alpha1->4 and alpha1->6 linkages from amylose/starch Azotobacter chroococcum ?
-
?
2.4.1.B34 additional information inactive on sucrose, panose, nigerose, beta-cyclodextrins, and isomalto-oligosaccharides with degree of polymerzation of 2, 3, and 5 Azotobacter chroococcum ?
-
?
2.4.1.B34 additional information GtfD shows clear hydrolase/transglycosylase activity with malto-oligosaccharides with degree of polymerzation of 3 to 7 and forms a range of shorter and longer oligosaccharides. The enzyme is unable to synthesize consecutive alpha1->6 glucosidic bonds. Instead, it forms a high molecular mass and branched alpha-glucan with alternating alpha1->4 and alpha1->6 linkages from amylose/starch Azotobacter chroococcum NCIMB 8003 ?
-
?
2.4.1.B34 additional information inactive on sucrose, panose, nigerose, beta-cyclodextrins, and isomalto-oligosaccharides with degree of polymerzation of 2, 3, and 5 Azotobacter chroococcum NCIMB 8003 ?
-
?
2.4.1.B34 potato starch
-
Azotobacter chroococcum ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.B34 ? x * 90000, SDS-PAGE, x * 86400, calculated Azotobacter chroococcum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B34 Achr_35950
-
Azotobacter chroococcum
2.4.1.B34 GtfD
-
Azotobacter chroococcum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B34 60
-
-
Azotobacter chroococcum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.B34 60
-
stable for at least 10 min Azotobacter chroococcum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B34 6.5
-
-
Azotobacter chroococcum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.B34 5.5 7 more than 70% of maximum activity Azotobacter chroococcum