Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Horchani, H.; Bussières, S.; Cantin, L.; Lhor, M.; Laliberté-Gemme, J.S.; Breton, R.; Salesse C.
    Enzymatic activity of lecithin:retinol acyltransferase: a thermostable and highly active enzyme with a likely mode of interfacial activation (2014), Biochim. Biophys. Acta, 1844, 1128-1836.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.135 SDS SDS is necessary for tLRAT extraction from cell lysates. tLRAT enzymatic activity drastically diminishes at SDS concentrations below 0.05% and remains unchanged when SDS concentration is increased from 0.05 to 1%. SDS is very important for tLRAT stability Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.135 additional information not inhibitory: retinyl heptanoate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.135 additional information
-
 additional information the water-soluble phospholipid:retinol molar ratio is very critical in the assay. It should be equal to at least 12.5:1 to allow proper solubilization of retinol by water-soluble phospholipid. A water-soluble phospholipid:tLRAT molar ratio of 4200000:1 should not be exceeded, otherwise a large decrease in activity occurs Homo sapiens
2.3.1.135 0.055
-
 all-trans-retinol pH not specified in the publication, 20°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.135 Homo sapiens O95237
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.135 SDS is necessary for tLRAT extraction from cell lysates. tLRAT enzymatic activity drastically diminishes at SDS concentrations below 0.05% and remains unchanged when SDS concentration is increased from 0.05 to 1%. SDS is very important for tLRAT stability. Detergents such as 0.2% Triton X-100, 0.7% CHAPSO,and 1.2 mM n-dodecyl-beta-D-maltoside and sodium are not effective Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.135 didecanoylphosphatidylcholine + all-trans-retinol
-
 Homo sapiens all-trans-retinyl decanoate + 2-decanoylglycerophosphocholine activity is about 10% of that with diheptanoylphosphatidylcholine ?
2.3.1.135 diheptanoylphosphatidylcholine + all-trans-retinol
-
 Homo sapiens all-trans-retinyl heptanoate + 2-heptanoylglycerophosphocholine
-
 ?
2.3.1.135 dilauroylphosphatidylcholine + all-trans-retinol
-
 Homo sapiens all-trans-retinyl laurate + 2-lauroylglycerophosphocholine
-
 ?
2.3.1.135 dilauroylphosphatidylcholine + all-trans-retinol
-
 Homo sapiens all-trans-retinyl laurate + 2-lauroylglycerophosphocholine activity is about 4% of that with diheptanoylphosphatidylcholine ?
2.3.1.135 dimyristoylphosphatidylcholine + all-trans-retinol activity is about 3% of that with diheptanoylphosphatidylcholine Homo sapiens all-trans-retinyl myristate + 2-myristoylglycerophosphocholine
-
 ?
2.3.1.135 dioctanoylphosphatidylcholine + all-trans-retinol activity is less than 15% of that with diheptanoylphosphatidylcholine Homo sapiens all-trans-retinyl octanoate + 2-octanoylglycerophosphocholine
-
 ?
2.3.1.135 dioleoylphosphatidylcholine + all-trans-retinol
-
 Homo sapiens all-trans-retinyl oleate + 2-oleoylglycerophosphocholine
-
 ?
2.3.1.135 dipalmitoylphosphatidylcholine + all-trans-retinol activity is about 2% of that with diheptanoylphosphatidylcholine Homo sapiens all-trans-retinyl palmitate + 2-palmitoylglycerophosphocholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.135 lecithin:retinol acyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.135 20 25
-
 Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.135 35
-
 15% of maximum activity Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.135 40.4
-
 all-trans-retinol pH not specified in the publication, 20°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.135 9.2
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.135 10
-
 80% of maximum activity Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.135 730
-
 all-trans-retinol pH not specified in the publication, 20°C Homo sapiens