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Literature summary extracted from

  • Norris, A.L.; Nickels, J.; Sokolov, A.P.; Serpersu, E.H.
    Protein dynamics are influenced by the order of ligand binding to an antibiotic resistance enzyme (2014), Biochemistry, 53, 30-38.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.81 0.0013
-
 tobramycin pH 7.6, 25°C Pseudomonas aeruginosa
2.3.1.81 0.0015
-
 sisomycin pH 7.6, 25°C Pseudomonas aeruginosa
2.3.1.81 0.0026
-
 kanamycin B pH 7.6, 25°C Pseudomonas aeruginosa
2.3.1.81 0.0048
-
 kanamycin A pH 7.6, 25°C Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.81 Pseudomonas aeruginosa Q51405
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.81 acetyl-CoA + kanamycin A
-
 Pseudomonas aeruginosa CoA + N3'-acetylkanamycin A
-
 ?
2.3.1.81 acetyl-CoA + kanamycin B
-
 Pseudomonas aeruginosa CoA + N3'-acetylkanamycin B
-
 ?
2.3.1.81 acetyl-CoA + neomycin
-
 Pseudomonas aeruginosa CoA + N3-acetylneomycin
-
 ?
2.3.1.81 acetyl-CoA + paromomycin
-
 Pseudomonas aeruginosa CoA + N3-acetylparamomycin
-
 ?
2.3.1.81 acetyl-CoA + ribostamycin
-
 Pseudomonas aeruginosa CoA + N3-acetylribostamycin
-
 ?
2.3.1.81 acetyl-CoA + sisomycin
-
 Pseudomonas aeruginosa CoA + N3'-acetylsisomycin
-
 ?
2.3.1.81 acetyl-CoA + tobramycin
-
 Pseudomonas aeruginosa CoA + N3'-acetyltobramycin
-
 ?
2.3.1.81 additional information isoform AAC-IIIb demonstrates fast antibiotic turnover rates, high antibiotic affinity. Aminoglycoside binding becomes more enthalpically favored and entropically disfavored when CoASH is complexed to AAC-IIIb. The enzyme catalytically and enthalpically favor those aminoglycosides with amine groups at the 2' carbon and show an increase in affinity of all aminoglycosides when CoASH is present Pseudomonas aeruginosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.81 Aac(3)-IIIb
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.81 10
-
 neomycin pH 7.6, 25°C Pseudomonas aeruginosa
2.3.1.81 24
-
 ribostamycin pH 7.6, 25°C Pseudomonas aeruginosa
2.3.1.81 34
-
 neomycin E pH 7.6, 25°C Pseudomonas aeruginosa
2.3.1.81 46
-
 sisomycin pH 7.6, 25°C Pseudomonas aeruginosa
2.3.1.81 48
-
 tobramycin pH 7.6, 25°C Pseudomonas aeruginosa
2.3.1.81 51
-
 kanamycin A pH 7.6, 25°C Pseudomonas aeruginosa
2.3.1.81 55
-
 kanamycin B pH 7.6, 25°C Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.81 10600
-
 kanamycin A pH 7.6, 25°C Pseudomonas aeruginosa
2.3.1.81 21200
-
 kanamycin B pH 7.6, 25°C Pseudomonas aeruginosa
2.3.1.81 30700
-
 sisomycin pH 7.6, 25°C Pseudomonas aeruginosa
2.3.1.81 36900
-
 tobramycin pH 7.6, 25°C Pseudomonas aeruginosa