EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | 4-chloromercuribenzoate | 1 mM, complete inactivation | Thermoanaerobacter brockii | |
1.1.1.2 | additional information | not inhibitory: EDTA (5 mM), MgCl2 (10 mM), CaCl2 (1O mM), CuCl2 (1 mM) and ZnCl2 (1 mM) | Thermoanaerobacter brockii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.2 | 0.12 | - |
Butanone | pH 7.8, 40°C | Thermoanaerobacter brockii | |
1.1.1.2 | 0.31 | - |
butan-2-ol | pH 7.8, 40°C | Thermoanaerobacter brockii | |
1.1.1.2 | 0.7 | - |
acetone | pH 7.8, 40°C | Thermoanaerobacter brockii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 40000 | - |
4 * 40000, SDS-PAGE | Thermoanaerobacter brockii |
1.1.1.2 | 150000 | - |
gel filtration | Thermoanaerobacter brockii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.2 | Thermoanaerobacter brockii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.2 | - |
Thermoanaerobacter brockii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.2 | acetone + NADPH + H+ | about 13% of the activity with butan-2-ol | Thermoanaerobacter brockii | propan-2-ol + NADP+ | - |
? | |
1.1.1.2 | butan-2-ol + NADP+ | good substrate | Thermoanaerobacter brockii | butanone + NADPH + H+ | - |
r | |
1.1.1.2 | butanone + NADPH + H+ | - |
Thermoanaerobacter brockii | butan-2-ol + NADP+ | - |
r | |
1.1.1.2 | cyclohexanol + NADP+ | about 15% of the activity with butan-2-ol | Thermoanaerobacter brockii | cyclohexanone + NADPH + H+ | - |
? | |
1.1.1.2 | additional information | activity of the reversible enzyme is highest for secondary alcohols and decreases in order of acetaldehyde, linear ketones, cyclic ketones and primary alcohols. It is active on five- to seven-membered ring ketones but not with cyclo-octanone as substrate | Thermoanaerobacter brockii | ? | - |
? | |
1.1.1.2 | pent-1-en-3-ol + NADP+ | good substrate | Thermoanaerobacter brockii | pent-1-en-3-one + NADPH + H+ | - |
? | |
1.1.1.2 | propan-2-ol + NADP+ | good substrate | Thermoanaerobacter brockii | acetone + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.2 | tetramer | 4 * 40000, SDS-PAGE | Thermoanaerobacter brockii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 65 | - |
70 min, no loss of activity | Thermoanaerobacter brockii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 7.8 | - |
- |
Thermoanaerobacter brockii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 7 | 9 | more than 50% of maximum activity | Thermoanaerobacter brockii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | additional information | no activity with NAD(H) | Thermoanaerobacter brockii | |
1.1.1.2 | NADPH | - |
Thermoanaerobacter brockii |