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Literature summary extracted from

  • Eniyan, K.; Bajpai, U.
    Cloning, expression, purification and bioinformatic analysis of 2-methylcitrate synthase from Mycobacterium tuberculosis (2015), Asian Pac. J. Trop. Med., 8, 19-23.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.5 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.5 soluble
-
 Mycobacterium tuberculosis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.5 42900
-
 x * 42900, calculated from amino acid sequence Mycobacterium tuberculosis
2.3.3.5 43000
-
 x * 43000, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate Mycobacterium tuberculosis
-
 (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate Mycobacterium tuberculosis H37Rv
-
 (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.5 Mycobacterium tuberculosis
-
-
-
2.3.3.5 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.5 Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate
-
 Mycobacterium tuberculosis (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate
-
 Mycobacterium tuberculosis H37Rv (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.5 ? x * 43000, SDS-PAGE Mycobacterium tuberculosis
2.3.3.5 ? x * 42900, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.3.5 Rv1131
-
 Mycobacterium tuberculosis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.3.5 Mycobacterium tuberculosis calculated from amino acid sequence
-
 8.6