EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.2 | synthesis | 50 microg of alcohol dehydrogenase AdhA, EC 1.1.1.2, and 50 microg actaldehyde dehydrogenase AldH, EC 1.2.1.10,in buffer solution (pH 8.0) containing NADPH, NADH and acetyl-CoA at 60°C, produce 1.6 mM ethanol from 3 mM acetyl-CoA after 90 min | Moorella sp. HUC22-1 |
1.2.1.10 | synthesis | 50 microg of alcohol dehydrogenase AdhA, EC 1.1.1.2, and 50 microg actaldehyde dehydrogenase AldH, EC 1.2.1.10, in buffer solution (pH 8.0) containing NADPH, NADH and acetyl-CoA at 60°C, produce 1.6 mM ethanol from 3 mM acetyl-CoA after 90 min | Moorella sp. HUC22-1 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.2 | expression in Escherichia coli | Moorella sp. HUC22-1 |
1.2.1.10 | expression in Escherichia coli | Moorella sp. HUC22-1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.2 | 10 | - |
acetaldehyde | isoform AdhA, pH 8.0, 60°C | Moorella sp. HUC22-1 | |
1.1.1.2 | 40 | - |
ethanol | isoform AdhA, pH 8.0, 60°C | Moorella sp. HUC22-1 | |
1.1.1.2 | 55.8 | - |
acetaldehyde | isoform AdhB, pH 8.0, 60°C | Moorella sp. HUC22-1 | |
1.2.1.10 | 1.6 | - |
acetyl-CoA | pH 8.0, 60°C | Moorella sp. HUC22-1 | |
1.2.1.10 | 4.3 | - |
acetaldehyde | pH 8.0, 60°C | Moorella sp. HUC22-1 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.2 | Moorella sp. HUC22-1 | - |
- |
- |
1.2.1.10 | Moorella sp. HUC22-1 | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 0.032 | - |
isoform AdhA, oxidation of ethanol, cofactor NAD+, pH 8.0, 60°C | Moorella sp. HUC22-1 |
1.1.1.2 | 0.049 | - |
isoform AdhA, reduction of acetaldehyde, cofactor NADH, pH 8.0, 60°C | Moorella sp. HUC22-1 |
1.1.1.2 | 2.9 | - |
isoform AdhB, reduction of acetaldehyde, cofactor NADPH, pH 8.0, 60°C | Moorella sp. HUC22-1 |
1.1.1.2 | 11.9 | - |
isoform AdhA, oxidation of ethanol, cofactor NADP+, pH 8.0, 60°C | Moorella sp. HUC22-1 |
1.1.1.2 | 50.4 | - |
isoform AdhA, reduction of acetaldehyde, cofactor NADPH, pH 8.0, 60°C | Moorella sp. HUC22-1 |
1.2.1.10 | 2.8 | - |
cleavage of thioester, cofactor NADPH, pH 8.0, 60°C | Moorella sp. HUC22-1 |
1.2.1.10 | 3.7 | - |
condensation of thioester, cofactor NADP+, pH 10.0, 60°C | Moorella sp. HUC22-1 |
1.2.1.10 | 18.8 | - |
cleavage of thioester, cofactor NADH, pH 8.0, 60°C | Moorella sp. HUC22-1 |
1.2.1.10 | 29.9 | - |
condensation of thioester, cofactor NAD+, pH 10.0, 60°C | Moorella sp. HUC22-1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.2 | acetaldehyde + NADPH + H+ | - |
Moorella sp. HUC22-1 | ethanol + NADP+ | - |
r | |
1.1.1.2 | butan-1-ol + NADP+ | isoform AdhA, 238% of the activity with acetaldehyde | Moorella sp. HUC22-1 | butanal + NADPH + H+ | - |
r | |
1.1.1.2 | butanal + NADPH + H+ | isoform AdhA, 625%, isoform AdhB, 108% of the activity with acetaldehyde | Moorella sp. HUC22-1 | butan-1-ol + NADP+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | isoform AdhB: no oxidation of ethanol | Moorella sp. HUC22-1 | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | isobutanol + NADP+ | isoform AdhA, 221% of the activity with acetaldehyde | Moorella sp. HUC22-1 | isobutyl aldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | isobutyl aldehyde + NADPH + H+ | isoform AdhA, 469%, isoform AdhB, 48% of the activity with acetaldehyde | Moorella sp. HUC22-1 | isobutanol + NADP+ | - |
r | |
1.1.1.2 | propan-1-ol + NADP+ | isoform AdhA, 162% of the activity with acetaldehyde | Moorella sp. HUC22-1 | propanal + NADPH + H+ | - |
r | |
1.1.1.2 | propanal + NADPH + H+ | isoform AdhA, 50%, isoform AdhB, 62% of the activity with acetaldehyde | Moorella sp. HUC22-1 | propan-1-ol + NADP+ | - |
r | |
1.2.1.10 | acetaldehyde + NAD+ + CoA | - |
Moorella sp. HUC22-1 | acetyl-CoA + NADH + H+ | - |
r | |
1.2.1.10 | acetaldehyde + NADP+ + CoA | - |
Moorella sp. HUC22-1 | acetyl-CoA + NADPH + H+ | - |
r | |
1.2.1.10 | acetyl-CoA + NADH + H+ | - |
Moorella sp. HUC22-1 | acetaldehyde + NAD+ + CoA | - |
r | |
1.2.1.10 | acetyl-CoA + NADPH + H+ | - |
Moorella sp. HUC22-1 | acetaldehyde + NADP+ + CoA | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.2 | AdhA | - |
Moorella sp. HUC22-1 |
1.1.1.2 | AdhB | - |
Moorella sp. HUC22-1 |
1.2.1.10 | ALDH | - |
Moorella sp. HUC22-1 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 60 | - |
at pH 8.0 | Moorella sp. HUC22-1 |
1.2.1.10 | 60 | - |
at pH 8.0 | Moorella sp. HUC22-1 |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 50 | - |
isoform AdhA, 84%, isoform AdhB, 26% of maximum activity | Moorella sp. HUC22-1 |
1.1.1.2 | 70 | - |
isoform AdhA, 61%, isoform AdhB 64% of maximum activity | Moorella sp. HUC22-1 |
1.2.1.10 | 50 | - |
71% of maximum activity | Moorella sp. HUC22-1 |
1.2.1.10 | 70 | - |
52% of maximum activity | Moorella sp. HUC22-1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 8 | - |
reduction of acetaldehyde | Moorella sp. HUC22-1 |
1.1.1.2 | 10 | - |
oxidation of ethanol | Moorella sp. HUC22-1 |
1.2.1.10 | 8 | - |
cleavage of thioester | Moorella sp. HUC22-1 |
1.2.1.10 | 10 | - |
condensation of thiopester | Moorella sp. HUC22-1 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | additional information | isoform AdhA, less than 1% activity with NAD(H), isoform AdhB, no activity with NAD(H) | Moorella sp. HUC22-1 | |
1.1.1.2 | NADPH | - |
Moorella sp. HUC22-1 | |
1.2.1.10 | NADH | NADH is preferred 7-8fold over NADPH | Moorella sp. HUC22-1 | |
1.2.1.10 | NADPH | NADH is preferred 7-8fold over NADPH | Moorella sp. HUC22-1 |