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Literature summary extracted from

  • Kim, M.; Kim, B.; Ahn, J.
    Biosynthesis of bioactive O-methylated flavonoids in Escherichia coli (2013), Appl. Microbiol. Biotechnol., 97, 7195-7204.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.231 DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli, functional coexpression of plant enzymes, e.g. 4-coumaroyl-CoA ligase and chalcone synthase, of different origins catalyzing steps in flavonoid biosynthesis pathways for production of naringenin from p-coumaric acid and synthesis of ponciretin and sakuranetin from naringenin in engineered and transformed Escherichia coli strain BL21(DE3) Glycine max
2.1.1.232 gene Os12g0240900, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli, functional coexpression of plant enzymes, e.g. 4-coumaroyl-CoA ligase and chalcone synthase, of different origins catalyzing steps in flavonoid biosynthesis pathways for production of naringenin from p-coumaric acid and synthesis of ponciretin and sakuranetin from naringenin in engineered and transformed Escherichia coli strain BL21(DE3) Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.231 S-adenosyl-L-methionine + naringenin Glycine max i.e. 4',5,7-trihyroxyflavanone, the enzyme transfers a methyl group to 4'-hydroxyl group of naringenin S-adenosyl-L-homocysteine + ponciretin i.e. 4'-methoxy-5,7-dihydroxyflavanone ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-naringenin Oryza sativa reaction of NOMT S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.231 Glycine max C6TAY1 cv. Paldal
-
2.1.1.232 Oryza sativa Q0IP69 cv. Nakdong, gene NOMT-2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.231 S-adenosyl-L-methionine + naringenin i.e. 4',5,7-trihyroxyflavanone, the enzyme transfers a methyl group to 4'-hydroxyl group of naringenin Glycine max S-adenosyl-L-homocysteine + ponciretin i.e. 4'-methoxy-5,7-dihydroxyflavanone ?
2.1.1.231 S-adenosyl-L-methionine + naringenin i.e. 4',5,7-trihyroxyflavanone, reaction of SOMT-2 Glycine max S-adenosyl-L-homocysteine + ponciretin i.e. 4'-methoxy-5,7-dihydroxyflavanone ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-naringenin reaction of NOMT Oryza sativa S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.231 SOMT2
-
Glycine max
2.1.1.231 soybean O-methyltransferase
-
Glycine max
2.1.1.232 naringenin O-methyltransferase
-
Oryza sativa
2.1.1.232 NOMT
-
Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.231 S-adenosyl-L-methionine
-
Glycine max
2.1.1.232 S-adenosyl-L-methionine
-
Oryza sativa

General Information

EC Number General Information Comment Organism
2.1.1.231 metabolism the enzyme catalyzes the last step in the biosynthesis of the bioactive flavonoid ponciretin, biosynthetic pathways starting with glucose, overview Glycine max
2.1.1.232 metabolism the enzyme catalyzes the last step in the biosynthesis of the bioactive flavonoid sakuranetin, biosynthetic pathway starting with glucose, overview Oryza sativa