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Literature summary extracted from

  • Yoo, M.H.; Yeom, S.J.; Park, C.S.; Lee, K.W.; Oh, D.K.
    Production of aglycon protopanaxadiol via compound K by a thermostable beta-glycosidase from Pyrococcus furiosus (2011), Appl. Microbiol. Biotechnol., 89, 1019-1028.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.B28 synthesis beta-glycosidase converts ginsenosides Rb1, Rb2, Rc, and Rd to protopanaxadiol aglycone via compound K. With increases in the enzyme activity, the productivities increase. The substrate concentration is optimal at ginsenoside Rd or 10% (w/v) ginseng root extract. 4 mM of ginsenoside Rd is converted to 3.3 mM compound K with a yield of 82.5% (mol/mol) and a productivity of 2010 mg per l and h at 1 h and is hydrolyzed completely to the aglycone with 364 mg per l and h after 5 h Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B28 55488
-
 x * 55500, SDS-PAGE, x * 55488, calculated Pyrococcus furiosus
3.2.1.B28 55500
-
 x * 55500, SDS-PAGE, x * 55488, calculated Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B28 Pyrococcus furiosus Q51723
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B28 0.08
-
 substrate ginsenoside Rc, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 0.55
-
 substrate ginsenoside Rb2, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 4.5
-
 substrate 4-nitrophenyl alpha-L-arabinofuranoside, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 4.5
-
 substrate ginsenoside Rb1, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 5.5
-
 substrate ginsenoside Rd, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 15
-
 substrate 4-nitrophenyl alpha-L-arabinopyranoside, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 529
-
 substrate 2-nitrophenyl beta-D-galactopyranoside, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 561
-
 substrate 4-nitrophenyl beta-D-galactopyranoside, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 828
-
 substrate 2-nitrophenyl beta-D-glucopyranoside, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 912
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 5.5, 95°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B28 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pyrococcus furiosus 2-nitrophenol + D-galactose
-
 ?
3.2.1.B28 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 2-nitrophenol + D-glucose
-
 ?
3.2.1.B28 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + L-arabinose
-
 ?
3.2.1.B28 4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + L-arabinose
-
 ?
3.2.1.B28 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-galactose
-
 ?
3.2.1.B28 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-glucose
-
 ?
3.2.1.B28 ginsenoside Rb1 + 4 H2O
-
 Pyrococcus furiosus protopanaxadiol aglycone + 4 D-glucopyranose
-
 ?
3.2.1.B28 ginsenoside Rb2 + 4 H2O
-
 Pyrococcus furiosus protopanaxadiol aglycone + 3 D-glucopyranose + L-arabinopyranose
-
 ?
3.2.1.B28 ginsenoside Rc + 4 H2O
-
 Pyrococcus furiosus protopanaxadiol aglycone + 3 D-glucopyranose + L-arabinopyranose
-
 ?
3.2.1.B28 ginsenoside Rd + 3 H2O
-
 Pyrococcus furiosus protopanaxadiol aglycone + 3 D-glucopyranose
-
 ?
3.2.1.B28 additional information enzyme converts ginsenosides Rb1, Rb2, Rc, and Rd to protopanaxadiol aglycone via compound K Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B28 ? x * 55500, SDS-PAGE, x * 55488, calculated Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B28 CelB
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B28 95
-
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B28 95
-
 half-life 68 h Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B28 5.5
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B28 5 6 more than 80% of maximum activity Pyrococcus furiosus